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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fong, Lai Yen Ng, Chin Theng Cheok, Zhi Li Mohd Moklas, Mohamad Aris Hakim, Muhammad Nazrul Ahmad, Zuraini |
| Description | Country affiliation: Malaysia Author Affiliation: Fong LY ( Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.); Ng CT ( Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.); Cheok ZL ( Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.); Mohd Moklas MA ( Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.); Hakim MN ( Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.); Ahmad Z ( Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia. Electronic address: zuraini@upm.edu.my.) |
| Abstract | BACKGROUND: Endothelial cell activation is characterized by increased endothelial permeability and increased expression of cell adhesion molecules (CAMs). This allows monocyte adherence and migration across the endothelium to occur and thereby initiates atherogenesis process. Asiatic acid is a major triterpene isolated from Centella asiatica (L.) Urban and has been shown to possess anti-oxidant, anti-hyperlipidemia and anti-inflammatory activities. PURPOSE: We aimed to investigate protective effects of asiatic acid on tumor necrosis factor- (TNF- )-induced endothelial cell activation using human aortic endothelial cells (HAECs). STUDY DESIGN: For cell viability assays, HAECs were treated with asiatic acid for 24 h. For other assays, HAECs were pretreated with various doses of asiatic acid (10-40 µM) for 6 h followed by stimulation with TNF- (10 ng/ml) for 6 h. METHODS: Fluorescein isothiocyanate (FITC)-dextran permeability assay was performed using commercial kits. Total protein expression of CAMs such as E-selectin, ICAM-1, VCAM-1 and PECAM-1 as well as phosphorylation of IκB- were determined using western blot. The levels of soluble form of CAMs were measured using flow cytometry. Besides, we also examined the effects of asiatic acid on U937 monocyte adhesion and monocyte migration in HAECs using fluorescent-based assays. RESULTS: Asiatic acid significantly suppressed endothelial hyperpermeability, increased VCAM-1 expression and increased levels of soluble CAMs (sE-selectin, sICAM-1, sVCAM-1 and sPECAM-1) triggered by TNF- . Neither TNF- nor asiatic acid affects PECAM-1 expression. However, asiatic acid did not inhibit TNF- -induced increased monocyte adhesion and migration. Interestingly, asiatic acid suppressed increased phosphorylation of IκB- stimulated by TNF- . CONCLUSION: These results suggest that asiatic acid protects against endothelial barrier disruption and this might be associated with the inhibition of NF-κB activation. We have demonstrated a novel protective role of asiatic acid on endothelial function. This reveals the possibility to further explore beneficial effects of asiatic acid on chronic inflammatory diseases that are initiated by endothelial cell activation. |
| File Format | HTM / HTML |
| ISSN | 09447113 |
| Issue Number | 2 |
| Volume Number | 23 |
| e-ISSN | 1618095X |
| Journal | Phytomedicine |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-02-15 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Complementary Therapies Endothelial Cells Drug Effects Endothelium, Vascular Cytology Pentacyclic Triterpenes Pharmacology Aorta Cell Adhesion Cell Movement Cell Survival E-selectin Metabolism Gene Expression Regulation Humans I-kappa B Proteins Intercellular Adhesion Molecule-1 Monocytes Nf-kappa B Phosphorylation Signal Transduction Tumor Necrosis Factor-alpha U937 Cells Vascular Cell Adhesion Molecule-1 Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Molecular Medicine Pharmacology Complementary and Alternative Medicine Pharmaceutical Science |
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