NDLI: Polydiacetylene liposome microarray toward influenza a virus detection: effect of target size on turn-on signaling.

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Polydiacetylene liposome microarray toward influenza a virus detection: effect of target size on turn-on signaling.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Seo, Sungbaek Lee, Jiseok Choi, Eun-Jin Kim, Eun-Ju Song, Jae-Young Kim, Jinsang
Description	Country affiliation: United States Author Affiliation: Seo S ( Macromolecular Science and Engineering, University of Michigan, Ann Arbor, MI 48109-2136, USA.)
Abstract	Target size effect on the sensory signaling intensity of polydiacetylene (PDA) liposome microarrays was systematically investigated. Influenza A virus M1 peptide and M1 antibody were selected as a probe-target pair. While red fluorescence from the PDA liposome microarrays was observed when the larger M1 antibody was used as a target, when the same M1 antibody was used as a probe to detect the smaller M1 peptide sensory signal did not appear. The results reveal that the intensity of the PDA sensory signal is mainly related to the steric repulsion between probe-target complexes not the strength of the probe-target binding force. Based on this finding, we devised a PDA sensory system that directly detects influenza A whole virus as a larger target, and confirmed the target size effect on the signaling efficiency of PDA.
File Format	HTM / HTML
ISSN	10221336
Issue Number	9
Volume Number	34
e-ISSN	15213927
Journal	Macromolecular Rapid Communications
Language	English
Publisher	Wiley
Publisher Date	2013-05-14
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Molecula Biology Discipline Biochemistry Biosensing Techniques Methods Influenza A Virus Chemistry Genetics Liposomes Microarray Analysis Polyacetylenes Polymers Dimyristoylphosphatidylcholine Rna Probes Rna, Viral Analysis Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Materials Chemistry Polymers and Plastics

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