NDLI: Polymer microarrays for high throughput discovery of biomaterials.

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Polymer microarrays for high throughput discovery of biomaterials.

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Polymer microarrays for high throughput discovery of biomaterials.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hook, Andrew L. Chang, Chien-Yi Yang, Jing Scurr, David J. Langer, Robert Anderson, Daniel G. Atkinson, Steve Williams, Paul Davies, Martyn C. Alexander, Morgan R.
Description	Author Affiliation: Hook AL ( Laboratory of Biophysics and Surface Analysis, University of Nottingham.)
Abstract	The discovery of novel biomaterials that are optimized for a specific biological application is readily achieved using polymer microarrays, which allows a combinatorial library of materials to be screened in a parallel, high throughput format (1). Herein is described the formation and characterization of a polymer microarray using an on-chip photopolymerization technique (2). This involves mixing monomers at varied ratios to produce a library of monomer solutions, transferring the solution to a glass slide format using a robotic printing device and curing with UV irradiation. This format is readily amenable to many biological assays, including stem cell attachment and proliferation, cell sorting and low bacterial adhesion, allowing the ready identification of 'hit' materials that fulfill a specific biological criterion (3-5). Furthermore, the use of high throughput surface characterization (HTSC) allows the biological performance to be correlated with physio-chemical properties, hence elucidating the biological-material interaction (6). HTSC makes use of water contact angle (WCA) measurements, atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). In particular, ToF-SIMS provides a chemically rich analysis of the sample that can be used to correlate the cell response with a molecular moiety. In some cases, the biological performance can be predicted from the ToF-SIMS spectra, demonstrating the chemical dependence of a biological-material interaction, and informing the development of hit materials (5,3).
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/3636
Journal	Journal of Visualized Experiments
Issue Number	59
Language	English
Publisher	MyJove Corp.
Publisher Date	2012-01-25
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Biocompatible Materials High-throughput Screening Assays Microarray Analysis Chemistry Mass Spectrometry Microscopy, Atomic Force Photochemical Processes Photoelectron Spectroscopy Spectrum Analysis, Raman Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

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