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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | White, Joshua J. Reeber, Stacey L. Hawkes, Richard Sillitoe, Roy V. |
| Description | Country affiliation: United States Author Affiliation: White JJ ( Department of Neuroscience, Albert Einstein College of Medicine, Yeshiva University, USA.) |
| Abstract | The repeated and well-understood cellular architecture of the cerebellum make it an ideal model system for exploring brain topography. Underlying its relatively uniform cytoarchitecture is a complex array of parasagittal domains of gene and protein expression. The molecular compartmentalization of the cerebellum is mirrored by the anatomical and functional organization of afferent fibers. To fully appreciate the complexity of cerebellar organization we previously refined a wholemount staining approach for high throughput analysis of patterning defects in the mouse cerebellum. This protocol describes in detail the reagents, tools, and practical steps that are useful to successfully reveal protein expression patterns in the adult mouse cerebellum by using wholemount immunostaining. The steps highlighted here demonstrate the utility of this method using the expression of zebrinII/aldolaseC as an example of how the fine topography of the brain can be revealed in its native three-dimensional conformation. Also described are adaptations to the protocol that allow for the visualization of protein expression in afferent projections and large cerebella for comparative studies of molecular topography. To illustrate these applications, data from afferent staining of the rat cerebellum are included. |
| File Format | HTM / HTML |
| e-ISSN | 1940087X |
| DOI | 10.3791/4042 |
| Journal | Journal of Visualized Experiments |
| Issue Number | 62 |
| Language | English |
| Publisher | MyJove Corp. |
| Publisher Date | 2012-04-05 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Brain Chemistry Cerebellum Anatomy & Histology Chemistry Immunohistochemistry Animals Metabolism Mice Research Support, Non-u.s. Gov't Video-audio Media |
| Content Type | Text |
| Resource Type | Article |
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