NDLI: Construction of microdrive arrays for chronic neural recordings in awake behaving mice.

Please wait, while we are loading the content...

Year: 2013 Issue: 82

Year: 2013 Issue: 81

Year: 2013 Issue: 80

Year: 2013 Issue: 79

Year: 2013 Issue: 78

Year: 2013 Issue: 77

Isolation, processing and analysis of murine gingival cells.

Preparation of pancreatic acinar cells for the purpose of calcium imaging, cell injury measurements, and adenoviral infection.

5/6th nephrectomy in combination with high salt diet and nitric oxide synthase inhibition to induce chronic kidney disease in the Lewis rat.

Use of in vivo imaging to monitor the progression of experimental mouse cytomegalovirus infection in neonates.

Construction of microdrive arrays for chronic neural recordings in awake behaving mice.

Optogenetic perturbation of neural activity with laser illumination in semi-intact drosophila larvae in motion.

Multi-scale modification of metallic implants with pore gradients, polyelectrolytes and their indirect monitoring in vivo.

Measuring growth and gene expression dynamics of tumor-targeted S. typhimurium bacteria.

Fabrication of spatially confined complex oxides.

Automated high-throughput behavioral analyses in zebrafish larvae.

A new murine model of endovascular aortic aneurysm repair.

Skin punch biopsy explant culture for derivation of primary human fibroblasts.

The resident-intruder paradigm: a standardized test for aggression, violence and social stress.

The synergistic effect of visible light and gentamycin on Pseudomona aeruginosa microorganisms.

Utilizing repetitive transcranial magnetic stimulation to improve language function in stroke patients with chronic non-fluent aphasia.

Isolation of Human Atrial Myocytes for Simultaneous Measurements of $Ca^{2+}$ Transients and Membrane Currents

A multiplexed luciferase-based screening platform for interrogating cancer-associated signal transduction in cultured cells.

Coupled assays for monitoring protein refolding in Saccharomyces cerevisiae.

Reconstitution of a Kv channel into lipid membranes for structural and functional studies.

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Technical aspects of the mouse aortocaval fistula.

Acquiring fluorescence time-lapse movies of budding yeast and analyzing single-cell dynamics using GRAFTS.

Differentiation of newborn mouse skin derived stem cells into germ-like cells in vitro.

Examination of Drosophila larval tracheal terminal cells by light microscopy.

The Goeckerman regimen for the treatment of moderate to severe psoriasis.

Design, fabrication, and experimental characterization of plasmonic photoconductive terahertz emitters.

The in ovo CAM-assay as a xenograft model for sarcoma.

Isolation of sensory neurons of Aplysia californica for patch clamp recordings of glutamatergic currents.

Whole mount immunofluorescent staining of the neonatal mouse retina to investigate angiogenesis in vivo.

Imaging of biological tissues by desorption electrospray ionization mass spectrometry.

Isolation, purification and labeling of mouse bone marrow neutrophils for functional studies and adoptive transfer experiments.

Genetic manipulation in Δku80 strains for functional genomic analysis of Toxoplasma gondii.

Printing thermoresponsive reverse molds for the creation of patterned two-component hydrogels for 3D cell culture.

The multiBac protein complex production platform at the EMBL.

Designing a bio-responsive robot from DNA origami.

Microfabrication of nanoporous gold patterns for cell-material interaction studies.

Technique and considerations in the use of 4x1 ring high-definition transcranial direct current stimulation (HD-tDCS).

Studying interactions of Staphylococcus aureus with neutrophils by flow cytometry and time lapse microscopy.

Recombineering homologous recombination constructs in Drosophila.

A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions

A comparative approach to characterize the landscape of host-pathogen protein-protein interactions.

Year: 2013 Issue: 76

Year: 2013 Issue: 75

Year: 2013 Issue: 74

Year: 2013 Issue: 73

Year: 2013 Issue: 72

Year: 2013 Issue: 71

Construction of microdrive arrays for chronic neural recordings in awake behaving mice.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chang, Eric H. Frattini, Stephen A. Robbiati, Sergio Huerta, Patricio T.
Description	Author Affiliation: Chang EH ( Lab of Immune and Neural Networks, Feinstein Institute for Medical Research, North Shore LIJ Health System.)
Abstract	State-of-the-art electrophysiological recordings from the brains of freely behaving animals allow researchers to simultaneously examine local field potentials (LFPs) from populations of neurons and action potentials from individual cells, as the animal engages in experimentally relevant tasks. Chronically implanted microdrives allow for brain recordings to last over periods of several weeks. Miniaturized drives and lightweight components allow for these long-term recordings to occur in small mammals, such as mice. By using tetrodes, which consist of tightly braided bundles of four electrodes in which each wire has a diameter of 12.5 µm, it is possible to isolate physiologically active neurons in superficial brain regions such as the cerebral cortex, dorsal hippocampus, and subiculum, as well as deeper regions such as the striatum and the amygdala. Moreover, this technique insures stable, high-fidelity neural recordings as the animal is challenged with a variety of behavioral tasks. This manuscript describes several techniques that have been optimized to record from the mouse brain. First, we show how to fabricate tetrodes, load them into driveable tubes, and gold-plate their tips in order to reduce their impedance from MΩ to KΩ range. Second, we show how to construct a custom microdrive assembly for carrying and moving the tetrodes vertically, with the use of inexpensive materials. Third, we show the steps for assembling a commercially available microdrive (Neuralynx VersaDrive) that is designed to carry independently movable tetrodes. Finally, we present representative results of local field potentials and single-unit signals obtained in the dorsal subiculum of mice. These techniques can be easily modified to accommodate different types of electrode arrays and recording schemes in the mouse brain.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/50470
Journal	Journal of Visualized Experiments
Issue Number	77
Language	English
Publisher	MyJove Corp.
Publisher Date	2013-07-05
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Behavior, Animal Physiology Brain Electrophysiology Instrumentation Microelectrodes Animals Electrophysiological Phenomena Mice Neurons Research Support, N.i.h., Extramural Video-audio Media
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in