NDLI: Measuring Spatial and Temporal $Ca^{2+}$ Signals in Arabidopsis Plants

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Measuring Spatial and Temporal $Ca^{2+}$ Signals in Arabidopsis Plants

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Measuring Spatial and Temporal $Ca^{2+}$ Signals in Arabidopsis Plants

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhu, Xiaohong Taylor, Aaron Zhang, Shenyu Zhang, Dayong Feng, Ying Liang, Gaimei Zhu, Jian-Kang
Description	Author Affiliation: Zhu X ( Department of Horticulture and Landscape Architecture, Purdue University); Taylor A ( Bindley Bioscience Center, Purdue University.); Zhang S ( Department of Horticulture and Landscape Architecture, Purdue University.); Zhang D ( Department of Horticulture and Landscape Architecture, Purdue University); Feng Y ( Department of Horticulture and Landscape Architecture, Purdue University); Liang G ( Department of Horticulture and Landscape Architecture, Purdue University); Zhu JK ( Department of Horticulture and Landscape Architecture, Purdue University)
Abstract	Developmental and environmental cues induce Ca(2+) fluctuations in plant cells. Stimulus-specific spatial-temporal Ca(2+) patterns are sensed by cellular Ca(2+) binding proteins that initiate Ca(2+) signaling cascades. However, we still know little about how stimulus specific Ca(2+) signals are generated. The specificity of a Ca(2+) signal may be attributed to the sophisticated regulation of the activities of Ca(2+) channels and/or transporters in response to a given stimulus. To identify these cellular components and understand their functions, it is crucial to use systems that allow a sensitive and robust recording of Ca(2+) signals at both the tissue and cellular levels. Genetically encoded Ca(2+) indicators that are targeted to different cellular compartments have provided a platform for live cell confocal imaging of cellular Ca(2+) signals. Here we describe instructions for the use of two Ca(2+) detection systems: aequorin based FAS (film adhesive seedlings) luminescence Ca(2+) imaging and case12 based live cell confocal fluorescence Ca(2+) imaging. Luminescence imaging using the FAS system provides a simple, robust and sensitive detection of spatial and temporal Ca(2+) signals at the tissue level, while live cell confocal imaging using Case12 provides simultaneous detection of cytosolic and nuclear Ca(2+) signals at a high resolution.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/51945
Journal	Journal of Visualized Experiments
Issue Number	91
Language	English
Publisher	MyJove Corp.
Publisher Date	2014-09-02
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Arabidopsis Metabolism Calcium Signaling Calcium Aequorin Chemistry Cytology Cell Nucleus Cytosol Luminescent Measurements Microscopy, Confocal Plants, Genetically Modified Seedling Research Support, N.i.h., Extramural Video-audio Media
Content Type	Text
Resource Type	Article

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