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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Faize, Mohamed Faize, Lydia Petri, Cesar Barba-Espin, Gregorio Diaz-Vivancos, Pedro Clemente-Moreno, María José Koussa, Tayeb Rifai, Lalla Aicha Burgos, Lorenzo Hernandez, José Antonio |
| Description | Country affiliation: Morocco Author Affiliation: Faize M ( Laboratory of Plant Biotechnology, Ecology and Ecosystem Valorization, Faculty of Sciences, University Chouaib Doukkali, 24000 El Jadida, Morocco.) |
| Abstract | In this study we examined the role of antioxidant metabolism in in vitro shoot multiplication. We generated transgenic plum plantlets overexpressing the cytsod and cytapx genes in cytosol under the control of the constitutive promoter CaMV35S. Three transgenic lines with up-regulated sod at transcriptional levels that showed silenced cytapx expression displayed an elevated in vitro multiplication rate. By contrast, a transgenic line harboring several copies of cytapx and with elevated APX enzymatic activity did not show any improvement in plant vigor, measured as the number of axillary shoots and shoot length. All of the lines with elevated micropropagation ability exhibited intensive H2O2 accumulation, monitored by 3,3'-diaminobenzidine (DAB) staining as well as by colorimetric analysis, providing direct in vitro evidence of the role of H2O2 and antioxidant genes in in vitro shoot multiplication. |
| File Format | HTM / HTML |
| ISSN | 01761617 |
| Issue Number | 7 |
| Volume Number | 170 |
| e-ISSN | 16181328 |
| Journal | Journal of Plant Physiology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-05-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Botany Ascorbate Peroxidases Metabolism Gene Expression Regulation, Enzymologic Genetics Hydrogen Peroxide Plant Shoots Enzymology Prunus Superoxide Dismutase Antioxidants Cytosol Gene Expression Gene Expression Regulation, Plant Analysis Isoenzymes Peas Plant Proteins Growth & Development Plants, Genetically Modified Spinacia Oleracea Transgenes Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Plant Science Agronomy and Crop Science |
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