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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Deng, Shiang-Lin Hsiao, Tai-Shen Shih, Ke-Ying Hong, Jin-Long |
| Description | Country affiliation: Taiwan Author Affiliation: Deng SL ( National Sun Yat-Sen University, Department of Materials and Optoelectric Science, Kaohsiung 80424, Taiwan.); Hsiao TS ( National Sun Yat-Sen University, Department of Materials and Optoelectric Science, Kaohsiung 80424, Taiwan.); Shih KY ( National Sun Yat-Sen University, Department of Materials and Optoelectric Science, Kaohsiung 80424, Taiwan.); Hong JL ( National Sun Yat-Sen University, Department of Materials and Optoelectric Science, Kaohsiung 80424, Taiwan. Electronic address: jlhong@mail.nsysu.edu.tw.) |
| Abstract | A fluorescent biological sensor utilizing aggregation-enhanced emission (AEE) property was developed in our laboratory. First, the AEE-active fluorescent tetraphenylthiophene (TP) unit was synthetically connected to poly(N-isopropylacrylamide) by covalent and ionic bonds, resulting in the respective c- and i-TP-PNIPAM for the detection and quantification of the bovine serum albumin (BSA) model protein. When bind to BSA, the ionic i-TP-PNIPAM shows much better fluorescence (FL) sensitivity compared to c-PNIPAM. The fluorescence (FL) intensity of i-TP-PNIAPM displays a good linear dependence on concentration of BSA (0-1 mg/mL), indicating quantitative fluorimetric protein detection can be achieved. Further addition of anionic surfactant of sodium dodecylsulfate (SDS) considerably raised the FL intensity of the complex solution. All the FL response was discussed in term of conformational freedom of the TP unit under different environmental constraints. |
| File Format | HTM / HTML |
| ISSN | 10111344 |
| Volume Number | 138 |
| e-ISSN | 18732682 |
| Journal | Journal of Photochemistry and Photobiology B: Biology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-09-05 |
| Publisher Place | Switzerland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biology Acrylic Resins Chemistry Fluorometry Serum Albumin, Bovine Analysis Thiophenes Metabolism Animals Anions Cations Cattle Circular Dichroism Protein Binding Sodium Dodecyl Sulfate Static Electricity Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Radiology, Nuclear Medicine and Imaging Biophysics Radiological and Ultrasound Technology Radiation |
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