| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Tenório, Denise P. L. A. Andrade, Camila G. Cabral Filho, Paulo E. Sabino, Caetano P. Kato, Ilka T. Carvalho, Luiz B. Alves, Severino Ribeiro, Martha S. Fontes, Adriana Santos, Beate S. |
| Description |
Country affiliation: Brazil Author Affiliation: Tenório DP ( Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Recife, PE, Brazil.); Andrade CG ( Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, Brazil.); Cabral Filho PE ( Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, PE, Brazil.); Sabino CP ( Centro de Lasers e Aplicações, IPEN-CNEN/SP, São Paulo, SP, Brazil.); Kato IT ( Centro de Lasers e Aplicações, IPEN-CNEN/SP, São Paulo, SP, Brazil.); Carvalho LB ( Laboratório de Imunopatologia Keizo Asami, Universidade Federal de Pernambuco, Recife, PE, Brazil.); Alves S ( Departamento de Química Fundamental, Universidade Federal de Pernambuco, Recife, PE, Brazil.); Ribeiro MS ( Centro de Lasers e Aplicações, IPEN-CNEN/SP, São Paulo, SP, Brazil.); Fontes A ( Departamento de Biofísica e Radiobiologia, Universidade Federal de Pernambuco, Recife, PE, Brazil. Electronic address: adriana.fontes.biofisica@gmail.com.); Santos BS ( Departamento de Ciências Farmacêuticas, Universidade Federal de Pernambuco, Recife, PE, Brazil. Electronic address: beate_santos@yahoo.com.br.) |
| Abstract |
Semiconductor colloidal quantum dots (QDs) have been applied in biological analysis due to their unique optical properties and their versatility to be conjugated to biomolecules, such as lectins and antibodies, acquiring specificity to label a variety of targets. Concanavalin A (Con A) lectin binds specifically to -d-mannose and -d-glucose regions of saccharides that are usually expressed on membranes of mammalian cells and on cell walls of microbials. Candida albicans is the most common fungal opportunistic pathogen present in humans. Therefore, in this work, this fungus was chosen as a model for understanding cells and biofilm-forming organisms. Here, we report an efficient bioconjugation process to bind CdTe (Cadmium Telluride) QDs to Con A, and applied the bioconjugates to label saccharide structures on the cellular surface of C. albicans suspensions and biofilms. By accomplishing hemagglutination experiments and circular dichroism, we observed that the Con A structure and biochemical properties were preserved after the bioconjugation. Fluorescence microscopy images of yeasts and hyphae cells, as well as biofilms, incubated with QDs-(Con A) showed a bright orange fluorescence profile, indicating that the cell walls were specifically labeled. Furthermore, flow cytometry measurements confirmed that over 93% of the yeast cells were successfully labeled by QD-(Con A) complex. In contrast, non-conjugated QDs or QDs-(inhibited Con A) do not label any kind of biological system tested, indicating that the bioconjugation was specific and efficient. The staining pattern of the cells and biofilms demonstrate that QDs were effectively bioconjugated to Con A with specific labeling of saccharide-rich structures on C. albicans. Consequently, this work opens new possibilities to monitor glucose and mannose molecules through fluorescence techniques, which can help to optimize phototherapy protocols for this kind of fungus. |
| File Format |
HTM / HTML |
| ISSN |
10111344 |
| Volume Number |
142 |
| e-ISSN |
18732682 |
| Journal |
Journal of Photochemistry and Photobiology B: Biology |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2015-01-01 |
| Publisher Place |
Switzerland |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Biology Candida Albicans Metabolism Concanavalin A Chemistry Fluorescent Dyes Glucose Analysis Mannose Quantum Dots Spectrometry, Fluorescence Cadmium Compounds Microscopy, Fluorescence Tellurium Thiomalates Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Radiology, Nuclear Medicine and Imaging Biophysics Radiological and Ultrasound Technology Radiation |
