NDLI: Amelogenin is phagocytized and induces changes in integrin configuration, gene expression and proliferation of cultured normal human dermal fibroblasts.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Almqvist, Sofia Werthén, Maria Johansson, Anna Agren, Magnus S. Thomsen, Peter Lyngstadaas, S. Petter
Description	Country affiliation: Sweden Author Affiliation: Almqvist S ( Department of Biomaterials, Sahlgrenska Academy at University of Gothenburg, Box 412, 405 30, Göteborg, Sweden. sofia.almqvist@biomaterials.gu.se)
Abstract	Fibroblasts are central in wound healing by expressing important mediators and producing and remodelling extracellular matrix (ECM) components. This study aimed at elucidating possible mechanisms of action of the ECM protein amelogenin on normal human dermal fibroblasts (NHDF). Amelogenin at 100 and 1000 microg/ml increased binding of NHDF via several integrins, including alphavbeta3, alphavbeta5 and alpha5beta1. Further, both surface interaction and cellular uptake of amelogenin by NHDF was observed using scanning and transmission electron microscopy. Gene microarray studies showed >8-fold up or down-regulation of genes, of which most are involved in cellular growth, migration and differentiation. The effect of amelogenin was exemplified by increased proliferation over 7 days. In conclusion, the beneficial effects of amelogenin on wound healing are possibly conducted by stimulating fibroblast signalling, proliferation and migration via integrin interactions. It is hypothesized that amelogenin stimulates wound healing by providing connective tissue cells with a temporary extracellular matrix.
File Format	HTM / HTML
ISSN	09574530
Issue Number	3
Volume Number	21
e-ISSN	15734838
Journal	Journal of Materials Science: Materials in Medicine
Language	English
Publisher	Springer
Publisher Date	2010-03-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biomedical Engineering Amelogenin Chemistry Fibroblasts Metabolism Integrins Phagocytosis Skin Cytology Cell Movement Cell Proliferation Cells, Cultured Extracellular Matrix Gene Expression Regulation Humans Microscopy, Electron, Scanning Methods Microscopy, Electron, Transmission Models, Biological Wound Healing Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Biomaterials Biophysics Bioengineering Biomedical Engineering

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