NDLI: Quantitative detection of Clostridium difficile in hospital environmental samples by real-time polymerase chain reaction.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mutters, R. Nonnenmacher, C. Susin, C. Albrecht, U. Kropatsch, R. Schumacher, S.
Spatial Coverage	Germany
Description	Country affiliation: Germany Author Affiliation: Mutters R ( Institute of Medical Microbiology and Hygiene, Philipps University Marburg, Marburg, Germany. mutters@staff.uni-marburg.de)
Abstract	C. difficile-associated diarrhoea occurs commonly in hospitals and is a significant cause of morbidity and mortality. Hospital surfaces are often contaminated with nosocomial pathogens and may be responsible for cross-transmission, especially if hardy Gram-positive and spore-forming organisms are involved. The aim of this study was to quantify C. difficile in the hospital environment near C. difficile-positive and -negative patients using a quantitative real-time polymerase chain reaction. A total of 531 samples was collected from the clinical environment and classified into three groups according to patient and ward status for C. difficile. As expected, there were significantly higher counts of C. difficile on the floor and in the near environment of C. difficile patients. However, a significant correlation was found between C. difficile counts on the floor and on the hands of patients and healthcare workers (HCWs) in wards without evidence of C. difficile. This suggests that asymptomatic carriage among patients and HCWs can also contribute towards C. difficile transmission in hospitals. In conclusion, C. difficile can be transmitted via personal contact or via contaminated areas of the hospital environment.
File Format	HTM / HTML
ISSN	01956701
Issue Number	1
Volume Number	71
e-ISSN	15322939
Journal	Journal of Hospital Infection
Language	English
Publisher	Elsevier
Publisher Date	2009-01-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Communicable Diseases Carrier State Epidemiology Clostridium Infections Clostridium Difficile Isolation & Purification Cross Infection Genetics Microbiology Fomites Germany Hospital Units Hospitals, University Humans Patients Personnel, Hospital Polymerase Chain Reaction Prevalence Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical)

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