NDLI: Prerequisite for highly efficient isoprenoid production by cyanobacteria discovered through the over-expression of 1-deoxy-d-xylulose 5-phosphate synthase and carbon allocation analysis.

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Prerequisite for highly efficient isoprenoid production by cyanobacteria discovered through the over-expression of 1-deoxy-d-xylulose 5-phosphate synthase and carbon allocation analysis.

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Prerequisite for highly efficient isoprenoid production by cyanobacteria discovered through the over-expression of 1-deoxy-d-xylulose 5-phosphate synthase and carbon allocation analysis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kudoh, Kai Kawano, Yusuke Hotta, Shingo Sekine, Midori Watanabe, Takafumi Ihara, Masaki
Description	Country affiliation: Japan Author Affiliation: Kudoh K ( Faculty of Agriculture, Shinshu University, 8403 Minamiminowa, Nagano 399-4511, Japan.); Kawano Y ( Faculty of Agriculture, Shinshu University, 8403 Minamiminowa, Nagano 399-4511, Japan); Hotta S ( Faculty of Agriculture, Shinshu University, 8403 Minamiminowa, Nagano 399-4511, Japan.); Sekine M ( Faculty of Agriculture, Shinshu University, 8403 Minamiminowa, Nagano 399-4511, Japan.); Watanabe T ( Faculty of Agriculture, Shinshu University, 8403 Minamiminowa, Nagano 399-4511, Japan.); Ihara M ( Faculty of Agriculture, Shinshu University, 8403 Minamiminowa, Nagano 399-4511, Japan)
Abstract	Cyanobacteria have recently been receiving considerable attention owing to their potential as photosynthetic producers of biofuels and biomaterials. Here, we focused on the production of isoprenoids by cyanobacteria, and aimed to provide insight into metabolic engineering design. To this end, we examined the over-expression of a key enzyme in 2-C-methyl-d-erythritol 4-phosphate (MEP) pathway, 1-deoxy-d-xylulose 5-phosphate synthase (DXS) in the cyanobacterium Synechocystis sp. PCC6803. In the DXS-over-expression strain (Dxs_ox), the mRNA and protein levels of DXS were 4-times and 1.5-times the levels in the wild-type (WT) strain, respectively. The carotenoid content of the Dxs_ox strain (8.4 mg/g dry cell weight [DCW]) was also up to 1.5-times higher than that in the WT strain (5.6 mg/g DCW), whereas the glycogen content dramatically decreased to an undetectable level. These observations suggested that the carotenoid content in the Dxs_ox strain was increased by consuming glycogen, which is a C-storage compound in cyanobacteria. We also quantified the total sugar (145 and 104 mg/g DCW), total fatty acids (31 and 24 mg/g DCW) and total protein (200 and 240 mg/g DCW) content in the WT and Dxs_ox strains, respectively, which were much higher than the carotenoid content. In particular, approximately 54% of the proteins were phycobiliproteins. This study demonstrated the major destinations of carbon flux in cyanobacteria, and provided important insights into metabolic engineering. Target yield can be improved through optimization of gene expression, the DXS protein stabilization, cell propagation depression and restriction of storage compound synthesis.
File Format	HTM / HTML
ISSN	13891723
Issue Number	1
Volume Number	118
e-ISSN	13474421
Journal	Journal of Bioscience and Bioengineering
Language	English
Publisher	Elsevier
Publisher Date	2014-07-01
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biomedical Engineering Discipline Microbiology Synechocystis Metabolism Terpenes Transferases Bacterial Proteins Analysis Carbohydrates Carbon Carotenoids Biosynthesis Erythritol Analogs & Derivatives Gene Expression Lipids Pentosephosphates Sugar Phosphates Genetics Ultrastructure Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Bioengineering Applied Microbiology and Biotechnology Biotechnology

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