NDLI: Synergism of regulatory elements in σ(B)- and σ(A)-dependent promoters enhances recombinant protein expression in Bacillus subtilis.

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Synergism of regulatory elements in σ(B)- and σ(A)-dependent promoters enhances recombinant protein expression in Bacillus subtilis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Phanaksri, Teva Luxananil, Plearnpis Panyim, Sakol Tirasophon, Witoon
Description	Country affiliation: Thailand Author Affiliation: Phanaksri T ( Institute of Molecular Biosciences, Mahidol University, Phutthamontol, Nakhon Pathom 73170, Thailand.); Luxananil P ( Microbial Cell Factory Laboratory, Bioresource Technology Unit, National Center for Genetic Engineering and Biotechnology, Thailand Scinece Park, Pathumthani 12120, Thailand.); Panyim S ( Institute of Molecular Biosciences, Mahidol University, Phutthamontol, Nakhon Pathom 73170, Thailand); Tirasophon W ( Institute of Molecular Biosciences, Mahidol University, Phutthamontol, Nakhon Pathom 73170, Thailand. Electronic address: witoon.tir@mahidol.ac.th.)
Abstract	Strong promoter is an essential factor for production of recombinant protein in various expression systems including Bacillus subtilis. In this study, we described a strategy to improve the expression efficiency using synthetic double promoter. Assembly of the conserved elements from σ(B)- and σ(A)-dependent promoters constitutively improved the yield of recombinant protein approximately 2-3-fold in both exponential and stationary growth phase. The synergistic effect in the double promoter was observed only when σ(B)-promoter was located upstream to σ(A)-promoter but independent to its orientation. A conserved element in either -10 or -35 box of σ(B)-promoter is sufficient to promote the synergism. Hence, this simple strategy of promoter engineering could be an effective way to generate a pool of strong constitutive promoters applicable for heterologous protein expression in B. subtilis in the future.
File Format	HTM / HTML
ISSN	13891723
Issue Number	4
Volume Number	120
e-ISSN	13474421
Journal	Journal of Bioscience and Bioengineering
Language	English
Publisher	Elsevier
Publisher Date	2015-10-01
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biomedical Engineering Discipline Microbiology Bacillus Subtilis Genetics Gene Expression Regulation, Bacterial Promoter Regions, Genetic Recombinant Proteins Biosynthesis Sigma Factor Base Sequence Conserved Sequence Analysis Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Bioengineering Applied Microbiology and Biotechnology Biotechnology

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