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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ohta, Erika Nakayama, Yasumune Mukai, Yukio Bamba, Takeshi Fukusaki, Eiichiro |
| Description | Country affiliation: Japan Author Affiliation: Ohta E ( Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.); Nakayama Y ( Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.); Mukai Y ( Department of Bioscience, Nagahama Institute of Bio-Science and Technology, 1266 Tamura-cho, Nagahama, Shiga 526-0829, Japan.); Bamba T ( Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.); Fukusaki E ( Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: fukusaki@bio.eng.osaka-u.ac.jp.) |
| Abstract | The budding yeast Saccharomyces cerevisiae is widely used for brewing and ethanol production. The ethanol sensitivity of yeast cells is still a serious problem during ethanol fermentation, and a variety of genetic approaches (e.g., random mutant screening under selective pressure of ethanol) have been developed to improve ethanol tolerance. In this study, we developed a strategy for improving ethanol tolerance of yeast cells based on metabolomics as a high-resolution quantitative phenotypic analysis. We performed gas chromatography-mass spectrometry analysis to identify and quantify 36 compounds on 14 mutant strains including knockout strains for transcription factor and metabolic enzyme genes. A strong relation between metabolome of these mutants and their ethanol tolerance was observed. Data mining of the metabolomic analysis showed that several compounds (such as trehalose, valine, inositol and proline) contributed highly to ethanol tolerance. Our approach successfully detected well-known ethanol stress related metabolites such as trehalose and proline thus, to further prove our strategy, we focused on valine and inositol as the most promising target metabolites in our study. Our results show that simultaneous deletion of LEU4 and LEU9 (leading to accumulation of valine) or INM1 and INM2 (leading to reduction of inositol) significantly enhanced ethanol tolerance. This study shows the potential of the metabolomic approach to identify target genes for strain improvement of S. cerevisiae with higher ethanol tolerance. |
| File Format | HTM / HTML |
| ISSN | 13891723 |
| Issue Number | 4 |
| Volume Number | 121 |
| e-ISSN | 13474421 |
| Journal | Journal of Bioscience and Bioengineering |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-04-01 |
| Publisher Place | Japan |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biomedical Engineering Discipline Microbiology Ethanol Metabolism Pharmacology Metabolome Metabolomics Saccharomyces Cerevisiae Drug Effects Fermentation Gas Chromatography-mass Spectrometry Inositol Genetics Mutation Phenotype Proline Chemistry Saccharomyces Cerevisiae Proteins Stress, Physiological Transcription Factors Trehalose Valine Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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