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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Di Bari, Gaetano Gentile, Eugenia Latronico, Tiziana Corriero, Giuseppe Fasano, Anna Marzano, Carlotta Nonnis Liuzzi, Grazia Maria |
| Description | Country affiliation: Italy Author Affiliation: Di Bari G ( Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona 4, 70126 Bari, Italy.); Gentile E ( Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona 4, 70126 Bari, Italy.); Latronico T ( Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona 4, 70126 Bari, Italy.); Corriero G ( Department of Biology, University of Bari, Via Orabona 4, 70126 Bari, Italy.); Fasano A ( Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona 4, 70126 Bari, Italy.); Marzano CN ( Department of Biology, University of Bari, Via Orabona 4, 70126 Bari, Italy.); Liuzzi GM ( Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari, Via Orabona 4, 70126 Bari, Italy. Electronic address: graziamaria.liuzzi@uniba.it.) |
| Abstract | Marine natural products extracted from sponges represent a new source for drug discovery. Here we describe a simple method for preparing aqueous extracts from 7 Mediterranean demosponges, which allowed the extraction of water-soluble compounds, such as proteins by homogenization of sponge tissue in phosphate buffered saline (PBS). The comparative analysis by SDS-PAGE showed differences in number of bands, bandwidth and intensity among the sponges analyzed. The PAS/silver staining revealed a substantial and different glycoprotein assortment among the demosponges studied. To further study the biological activities present in the sponge extracts, we determined the non-cytotoxic doses on four different mammalian cell types demonstrating that the optimal non-cytotoxic doses were cell type- and extract-dependent. In conclusion, the extraction method described in this paper represents a fast and efficient procedure for the extraction of water-soluble proteins from marine sponges. Furthermore, the cell viability data suggest the feasibility of this method for the direct in vitro cell-based assays. |
| File Format | HTM / HTML |
| ISSN | 13826689 |
| Issue Number | 3 |
| Volume Number | 38 |
| e-ISSN | 18727077 |
| Journal | Environmental Toxicology and Pharmacology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-11-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Environmental Health Discipline Pharmacology Astrocytes Drug Effects Electrophoresis, Polyacrylamide Gel Methods Porifera Chemistry Proteins Analysis Animals Biological Products Pharmacology Caco-2 Cells Cell Survival Cells, Cultured Cricetinae Glycoproteins Humans Maximum Tolerated Dose Classification Rats Solubility Comparative Study Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Health, Toxicology and Mutagenesis Medicine Toxicology Pharmacology |
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