NDLI: Demonstration of an outer membrane protein that is antigenically specific for Acinetobacter baumannii.

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Demonstration of an outer membrane protein that is antigenically specific for Acinetobacter baumannii.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Islam, Abul Hasnat Mohammad Shafiqul Singh, Kirnpal-Kaur Banga Ismail, Asma
Description	Country affiliation: Malaysia Author Affiliation: Islam AH ( Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia.)
Abstract	Acinetobacter baumannii is an emerging nosocomial pathogen that is resistant to many types of antibiotics, and hence, a fast, sensitive, specific, and economical test for its rapid diagnosis is needed. Development of such a test requires a specific antigen, and outer membrane proteins (OMPs) are the prime candidates. The goal of this study was to find a specific OMP of A. baumannii and demonstrate the presence of specific IgM, IgA, and IgG against the candidate protein in human serum. OMPs of A. baumannii ATCC 19606 and 16 other clinical isolates of A. baumannii were extracted from an overnight culture grown at 37 °C. Protein profiles were obtained using sodium dodecyl sulfate polyacrylamide gel electrophoresis, and Western blot analysis was performed to detect the presence of IgM, IgA, and IgG against the OMP in host serum. An antigenic 34.4-kDa OMP was uniquely recognized by IgM, IgA, and IgG from patients with A. baumannii infection, and it did not cross-react with sera from patients with other types of infection. The band was also found in the other 16 A. baumannii isolates. This 34.4-kDa OMP is a prime candidate for development of a diagnostic test for the presence of A. baumannii.
File Format	HTM / HTML
ISSN	07328893
Issue Number	1
Volume Number	69
e-ISSN	18790070
Journal	Diagnostic Microbiology and Infectious Disease
Language	English
Publisher	Elsevier
Publisher Date	2011-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Discipline Communicable Diseases Acinetobacter Infections Diagnosis Acinetobacter Baumannii Immunology Isolation & Purification Antibodies, Bacterial Blood Antigens, Bacterial Bacterial Outer Membrane Proteins Bacteriological Techniques Methods Microbiology Chemistry Humans Immunoassay Immunoglobulin A Immunoglobulin G Immunoglobulin M Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical)

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