NDLI: Real-time PCR for detection of Streptococcus suis serotype 2 in cerebrospinal fluid of human patients with meningitis.

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Real-time PCR for detection of Streptococcus suis serotype 2 in cerebrospinal fluid of human patients with meningitis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Nga, Tran Vu Thieu Nghia, Ho Dang Trung Tu, Le Thi Phuong Diep, To Song Mai, Nguyen Thi Hoang Chau, Tran Thi Hong Sinh, Dinh Xuan Phu, Nguyen Hoan Nga, Tran Thi Thu Chau, Nguyen Van Vinh Campbell, James Hoa, Ngo Thi Chinh, Nguyen Tran Hien, Tran Tinh Farrar, Jeremy Schultsz, Constance
Spatial Coverage	Vietnam
Description	Country affiliation: Vietnam Author Affiliation: Nga TV ( Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam.)
Abstract	Streptococcus suis serotype 2 is an emerging zoonotic pathogen and is the main cause of acute bacterial meningitis in adult patients in Vietnam. We developed an internally controlled real-time PCR for detection of S. suis serotype 2 in cerebrospinal fluid (CSF) samples targeted at the cps2J gene. Sensitivity and specificity in culture-confirmed clinical samples were 100%. The PCR detected S. suis serotype 2 infection in 101 of 238 (42.4%) prospectively collected CSF samples, of which 55 (23%) were culture positive. Culture-negative but PCR-positive CSF samples were significantly associated with the use of antimicrobial agents before admission. S. suis serotype 2 infection was more common than infections with Streptococcus pneumoniae and Neisseria meningitidis combined. Our results strikingly illustrate the additional diagnostic value of PCR in patients who are pretreated with antimicrobial agents and demonstrate the extremely high prevalence of S. suis infections among Vietnamese adult patients with bacterial meningitis.
File Format	HTM / HTML
ISSN	07328893
e-ISSN	18790070
DOI	10.1016/j.diagmicrobio.2010.12.015
Journal	Diagnostic Microbiology and Infectious Disease
Issue Number	4
Volume Number	70
Language	English
Publisher	Elsevier
Publisher Date	2011-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Microbiology Discipline Communicable Diseases Cerebrospinal Fluid Microbiology Meningitis, Bacterial Diagnosis Epidemiology Polymerase Chain Reaction Streptococcal Infections Streptococcus Suis Isolation & Purification Bacteriological Techniques Sensitivity And Specificity Vietnam Comparative Study Evaluation Studies Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical)

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