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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Doseeva, Victoria Forbes, Thomas Wolff, John Khripin, Yuri O'Neil, Dominic Rothmann, Thomas Nazarenko, Irina |
| Description | Country affiliation: United States Author Affiliation: Doseeva V ( QIAGEN North America, Research and Development, QIAGEN Inc., Gaithersburg, MD 20878, USA. victoria.doseeva@qiagen.com) |
| Abstract | Thermophilic helicase dependent amplification (tHDA), which employs helicase to unwind double-stranded DNA at constant temperature, is a relatively new isothermal nucleic acid amplification technology. In this study, the development and optimization of a 4-plex tHDA assay for detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) are described. tHDA is combined with sequence-specific sample preparation on magnetic beads and homogeneous endpoint fluorescence detection using dual-labeled probes. This 4-plex tHDA assay was applied to the detection of 2 genes on CT and a multicopy gene on NG in the presence of an internal control. The assay showed high analytical sensitivity and specificity of simultaneous CT/NG detection and is compatible with a wide variety of sample types and media. The isothermal reaction conditions and homogeneous endpoint detection utilized in this assay are well suited for laboratory automation and high-throughput screening applications as well as for point-of-care testing. |
| File Format | HTM / HTML |
| ISSN | 07328893 |
| Issue Number | 4 |
| Volume Number | 71 |
| e-ISSN | 18790070 |
| Journal | Diagnostic Microbiology and Infectious Disease |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-12-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Discipline Communicable Diseases Chlamydia Infections Diagnosis Chlamydia Trachomatis Isolation & Purification Dna Helicases Gonorrhea Molecular Diagnostic Techniques Methods Neisseria Gonorrhoeae Nucleic Acid Amplification Techniques Bacteriological Techniques Microbiology Enzymology Genetics Metabolism Humans Microspheres Oligonucleotide Probes Point-of-care Systems Sensitivity And Specificity Evaluation Studies Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Microbiology (medical) |
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