NDLI: Validation of the EntericBio Panel II® multiplex polymerase chain reaction system for detection of Campylobacter spp., Salmonella spp., Shigella spp., and verotoxigenic E. coli for use in a clinical diagnostic setting.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Koziel, Monika Corcoran, Dan O'Callaghan, Isabelle Sleator, Roy D. Lucey, Brigid
Spatial Coverage	Ireland
Description	Country affiliation: Ireland Author Affiliation: Koziel M ( Department of Biological Sciences, Cork Institute of Technology, Bishopstown, Cork, Ireland.)
Abstract	A total of 717 faeces samples were tested prospectively using the EntericBio Panel II® detection system (Serosep, Limerick, Ireland), in parallel with routine laboratory testing, which combines the EntericBio® system with retrospective culture of each specimen where a target is detected. Discrepancy analysis was conducted using molecular methods. The EntericBio Panel II® assay produced 585 negative and 132 positive results, namely, Campylobacter spp. (n = 66); SLT 1 and/or SLT 2 (n = 64); Salmonella spp. (n = 5); and Shigella spp. (n = 0). Three samples were positive for more than 1 target. Of these results, 4 Campylobacter spp. detections and 4 SLT 1/ SLT 2 detections remained unconfirmed, and the system failed to detect 2 Campylobacter spp. targets detected by routine laboratory detection. The sensitivity, specificity, positive predictive value, negative predictive value, and efficiency were calculated to be 98.4%, 98.7%, 93.9%, 99.7%, and 98.6%, respectively.
File Format	HTM / HTML
ISSN	07328893
Issue Number	1
Volume Number	75
e-ISSN	18790070
Journal	Diagnostic Microbiology and Infectious Disease
Language	English
Publisher	Elsevier
Publisher Date	2013-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Discipline Communicable Diseases Campylobacter Isolation & Purification Enterobacteriaceae Infections Diagnosis Molecular Diagnostic Techniques Methods Multiplex Polymerase Chain Reaction Salmonella Shiga-toxigenic Escherichia Coli Shigella Genetics Microbiology Feces Humans Ireland Predictive Value Of Tests Prospective Studies Sensitivity And Specificity Evaluation Studies Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical)

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