NDLI: Rapid simultaneous identification and quantitation of Staphylococcus aureus and Pseudomonas aeruginosa directly from bronchoalveolar lavage specimens using automated microscopy.

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Rapid simultaneous identification and quantitation of Staphylococcus aureus and Pseudomonas aeruginosa directly from bronchoalveolar lavage specimens using automated microscopy.

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Rapid simultaneous identification and quantitation of Staphylococcus aureus and Pseudomonas aeruginosa directly from bronchoalveolar lavage specimens using automated microscopy.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Metzger, Steven Frobel, Rachel A. Dunne, W. Michael
Description	Author Affiliation: Metzger S ( Accelerate Diagnostics Inc., Tucson, AZ 85714, USA. Electronic address: smetzger@axdx.com.); Frobel RA ( Washington University School of Medicine, St Louis, MO 63110, USA.); Dunne WM ( Washington University School of Medicine, St Louis, MO 63110, USA.)
Abstract	Diagnosis of ventilator-assisted pneumonia (VAP) requires pathogen quantitation of respiratory samples. Current quantitative culture methods require overnight growth, and pathogen identification requires an additional step. Automated microscopy can perform rapid simultaneous identification and quantitation of live, surface-immobilized bacteria extracted directly from patient specimens using image data collected over 3 h. Automated microscopy was compared to 1 µL loop culture and standard identification methods for Staphylococcus aureus and Pseudomonas spp. in 53 remnant bronchoalveolar lavage specimens. Microscopy identified 9/9 S. aureus and 7/7 P. aeruginosa in all specimens with content above the VAP diagnostic threshold. Concordance for specimens containing targets above the diagnostic threshold was 13/16, with concordance for sub-diagnostic content of 86/90. Results demonstrated that automated microscopy had higher precision than 1 µL loop culture (range ~0.55 log versus ≥1 log), with a dynamic range of ~4 logs (~10(3) to 10(6) CFU/mL).
File Format	HTM / HTML
ISSN	07328893
Issue Number	2
Volume Number	79
e-ISSN	18790070
Journal	Diagnostic Microbiology and Infectious Disease
Language	English
Publisher	Elsevier
Publisher Date	2014-06-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Discipline Communicable Diseases Automation, Laboratory Methods Bronchoalveolar Lavage Fluid Microbiology Microscopy Pneumonia, Ventilator-associated Diagnosis Pseudomonas Infections Staphylococcal Infections Humans Pseudomonas Aeruginosa Isolation & Purification Staphylococcus Aureus Evaluation Studies Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical)

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