NDLI: Detection of high-risk human papillomavirus infection in tonsillar specimens using 2 commercially available assays.

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Detection of high-risk human papillomavirus infection in tonsillar specimens using 2 commercially available assays.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cockerill, Cara C. Orvidas, Laura J. Moore, Eric J. Binnicker, Matthew J. Duresko, Brian J. Espy, Mark J. Cockerill, Franklin R. Tombers, Nicole M. Pritt, Bobbi S.
Description	Author Affiliation: Cockerill CC ( The Department of Otorhinolaryngology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Cockerill.cara@mayo.edu.); Orvidas LJ ( The Department of Otorhinolaryngology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Orvidas.laura@mayo.edu.); Moore EJ ( The Department of Otorhinolaryngology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Moore.eric@mayo.edu.); Binnicker MJ ( The Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Binnicker.Matthew@mayo.edu.); Duresko BJ ( The Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Duresko.brian@mayo.edu.); Espy MJ ( The Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Espy.mark@mayo.edu.); Cockerill FR ( The Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Franklincockerill@yahoo.com.); Tombers NM ( The Department of Otorhinolaryngology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Tombers.nicole@mayo.edu.); Pritt BS ( The Department of Laboratory Medicine and Pathology, Mayo Clinic, 200 First Street SW, Rochester, MN 55905. Electronic address: Pritt.Bobbi@mayo.edu.)
Abstract	THE OBJECTIVE OF THE STUDY IS TO DETERMINE THE PREVALENCE OF HIGH-RISK HUMAN PAPILLOMAVIRUS (HRHPV) INFECTION IN TONSILLAR SWABS AND TISSUE: Patients undergoing tonsillectomy for nonmalignant causes were enrolled. A flocked swab and fresh tissue were collected from the left and right tonsil of each patient. Specimens were tested for hrHPV DNA using the Roche cobas test and for the presence of E6/E7 messenger RNA using the Hologic Aptima hrHPV test. Of the 193 patients enrolled, 129 were in the pediatric group (ages 1-12years; median, 5years), and 64 were in the adult group (ages 13-55; median, 22years). All swab and tissue specimens were negative for hrHPV by both methods. Positive, negative, and internal controls performed as expected. We found a 0% rate of infection indicating that detectable hrHPV infection in tonsillar tissue appears to be uncommon in the children and adults in the population sampled.
File Format	HTM / HTML
ISSN	07328893
Issue Number	4
Journal	Diagnostic Microbiology and Infectious Disease
Volume Number	86
e-ISSN	18790070
Language	English
Publisher	Elsevier
Publisher Date	2016-12-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Discipline Communicable Diseases
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical)

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