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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Werren, John H. Loehlin, David W. Giebel, Jonathan D. |
| Description | Country affiliation: United States Author Affiliation: Werren JH ( Department of Biology, University of Rochester, Rochester, NY 14627, USA. werr@mail.rochester.edu) |
| Abstract | Nasonia is a complex of four closely related species of wasps with several features that make it an excellent system for a variety of genetic studies. These include a short generation time, ease of rearing, interfertile species, visible and molecular markers, and a sequenced genome. Furthermore, its parasitoid lifestyle allows investigations of questions relating to parasitoid/host dynamics, host preference, and specialist versus generalist biology. It also can serve as a behavior model for studies of courtship, male aggression and territoriality, female dispersal, and sex ratio control. This protocol describes a method to use RNA interference (RNAi) to knock down genes in Nasonia larvae. Unlike in Drosophila, RNAi in Nasonia is systemic. In the example presented here, adult red-eye-color phenotypes are produced by injecting double-stranded RNA (dsRNA) against the eye color gene cinnabar into last-instar Nasonia larvae. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| e-ISSN | 15596095 |
| DOI | 10.1101/pdb.prot5311 |
| Journal | Cold Spring Harbor Protocols |
| Issue Number | 10 |
| Volume Number | 2009 |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2009-10-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Clinical Laboratory Techniques Biology Rna Interference Wasps Genetics Physiology Animals Eye Color Genome Mutation Phenotype Species Specificity Research Support, N.i.h., Extramural |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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