NDLI: Chromosome banding of mitotic chromosomes from Drosophila larval brain.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pimpinelli, Sergio Bonaccorsi, Silvia Fanti, Laura Gatti, Maurizio
Abstract	The classical chromosome-banding techniques developed for mammalian chromosomes do not differentiate the euchromatic arms of Drosophila mitotic chromosomes. However, some of these techniques produce a sharp and highly reproducible banding of Drosophila heterochromatin. For example, the use of quinacrine-, Hoechst-, and N-banding differentiates Drosophila heterochromatin into 61 cytological entities, allowing precise localization of heterochromatic breakpoints. These banding techniques can also be successfully used to differentiate mitotic heterochromatin of various Drosophila and mosquito species. Here we present protocols routinely used in our laboratories for chromosome banding, including the use of Hoechst, 4',6-diamidino-2-phenylindole (DAPI), quinacrine, and Giemsa stains.
File Format	HTM / HTML
ISSN	19403402
Issue Number	3
Volume Number	2010
e-ISSN	15596095
Journal	Cold Spring Harbor Protocols
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2010-03-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Clinical Laboratory Techniques Chromosome Banding Methods Chromosomes Drosophila Cytology Mitosis Animals Brain Coloring Agents Fluorescent Dyes Larva Journal Article
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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Chromosome banding of mitotic chromosomes from Drosophila larval brain.