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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Rio, Donald C. Ares, Manuel Hannon, Gregory J. Nilsen, Timothy W. |
| Abstract | This protocol describes a method for RNA purification by sodium dodecyl sulfate (SDS) solubilization and phenol extraction. It is of wide utility and is used routinely to deproteinize RNAs in biological material that has been solubilized in SDS, an ionic detergent that dissolves membranes, disrupts protein-nucleic acid interactions, and inactivates ribonucleases. Once solubilized, addition of phenol or phenol:chloroform:isoamyl alcohol (PCA) completely denatures the protein, and it becomes insoluble in aqueous solution. PCA extraction is the method of choice for preparing cytoplasmic RNA from tissue culture cells or in any other situation (e.g., enzyme reactions) where solubilization in SDS is easily achievable. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| Issue Number | 6 |
| Journal | Cold Spring Harbor Protocols |
| Volume Number | 2010 |
| e-ISSN | 15596095 |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2010-06-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Molecular Biology Methods Phenol Chemistry Rna Isolation & Purification Sodium Dodecyl Sulfate Animals Solubility Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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