NDLI: Isolation of DNA from red blood cells in Xenopus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sive, Hazel L. Grainger, Robert M. Harland, Richard M.
Abstract	The amphibian Xenopus laevis is an important model organism that is particularly valuable for studies of early vertebrate development. Genomic DNA constitutes the total genetic information of an organism and it is used for Southern blotting, for determining gene structure, and for detecting the presence or absence of genes of interest. Genomic DNA can be extracted from Xenopus red blood cells, which are unlike the mammalian equivalent in that they contain nuclei. This article describes a protocol for the isolation of genomic DNA from frog red blood cells.
File Format	HTM / HTML
ISSN	19403402
Issue Number	1
Journal	Cold Spring Harbor Protocols
Volume Number	2012
e-ISSN	15596095
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2012-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Clinical Laboratory Techniques Dna Isolation & Purification Erythrocytes Xenopus Laevis Animals Molecular Biology Methods Journal Article
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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Isolation of DNA from red blood cells in Xenopus.