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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Stern, David L. Sucena, Elio |
| Abstract | The Drosophila cuticle carries a rich array of morphological details. Thus, cuticle examination has had a central role in the history of genetics. To prepare fine 'museum-quality,' permanent slides, it is best to mount specimens in Canada Balsam. It is difficult to give precise recipes for Canada Balsam, because every user seems to prefer a slightly different viscosity. Dilute solutions spread easily and do not dry too rapidly while mounting specimens. The disadvantage is that there is actually less Balsam in a 'drop' of the solution, and when dried, it can contract from the sides of the coverslip, sometimes disturbing the specimen. Unfortunately, there is no substitute for experience when using Canada Balsam. This protocol describes a procedure for mounting adult cuticles in Canada Balsam. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| Issue Number | 3 |
| Journal | Cold Spring Harbor Protocols |
| Volume Number | 2012 |
| e-ISSN | 15596095 |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2012-03-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Balsams Drosophila Anatomy & Histology Ultrastructure Microscopy Methods Preservation, Biological Specimen Handling Animals Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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