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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ross, William N. Manita, Satoshi |
| Abstract | Here we describe the use of wide-field charge-coupled device (CCD) camera-based imaging methods to detect the spatial and temporal aspects of calcium release from internal stores in dendrites of neurons in brain slice preparations. This approach is useful for revealing aspects of this signaling system, which is generally invisible to electrical recording. The changes in intracellular calcium ion concentrations, $[Ca^{2+}]_{i},$ sometimes occur as large-amplitude, propagating $Ca^{2+}$ waves or as much smaller, localized events (sparks). In this protocol, a cell is loaded with an indicator that responds to $Ca^{2+},$ waves or sparks are stimulated in the cell, and the spatial and temporal characteristics of calcium release from internal stores in the cell are detected using wide-field CCD camera-based imaging. Such camera systems have some advantages for detecting and analyzing these $[Ca^{2+}]_{i}$ changes because the waves are spatially extended and the sparks do not always occur at the same locations. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| Issue Number | 10 |
| Journal | Cold Spring Harbor Protocols |
| Volume Number | 2012 |
| e-ISSN | 15596095 |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2012-10-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Calcium Signaling Image Cytometry Methods Neurons Physiology Animals Brain Cytology Image Processing, Computer-assisted Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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