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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bayne, Lauren J. Vonderheide, Robert H. |
| Description | Country affiliation: United States Author Affiliation: Bayne LJ ( Abramson Family Cancer Research Institute, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104, USA.) |
| Abstract | This protocol describes a procedure for the evaluation of immune cells at the histological level. This technique preserves tissue architecture, making use of tissues that have been frozen in optimal cutting temperature (OCT) medium immediately after dissection and then cut with a cryostat into thin (â¼8 µm) sections. The tissue slices are transferred to microscope slides and, as described here, are fixed with either methanol or formaldehyde. The fixed tissue sections are then stained with antibodies directed against cell surface markers expressed by the immune cells of interest. The antibodies are detected with an avidin-biotin-based peroxidase system, and, after mounting, the sections can be stored and viewed for years. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| Issue Number | 9 |
| Volume Number | 2013 |
| e-ISSN | 15596095 |
| Journal | Cold Spring Harbor Protocols |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2013-09-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Immunohistochemistry Methods Immunophenotyping Neoplasms Immunology Pathology Animals Mice Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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