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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Mandemakers, Wim |
| Description | Country affiliation: Belgium Author Affiliation: Mandemakers W ( Laboratory for the Research of Neurodegenerative Diseases, VIB Center for the Biology of Disease, KU Leuven Center for Human Genetics, 3000 Leuven, Belgium.) |
| Abstract | Immunopanning of viable neurons requires availability of an antibody directed against a neuronal surface epitope that is specific for a neuron type of interest (e.g., corticospinal motor neurons [CSMNs]) and that is able to immobilize only neurons of interest from a dissociated brain suspension on a culture dish. However, few, if any, neuron type-specific monoclonal antibodies have been developed. Panning antibodies have not been characterized for CSMNs. To circumvent this issue, we developed a method to retrograde label CSMNs with a cholera toxin B subunit (CTB) tracer that localizes to the plasma membrane only of labeled CSMNs. The main objective of this procedure is to provide CSMNs with a surface marker that can be used to immunopan only the labeled CSMNs by using a CTB-specific antibody. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| Issue Number | 4 |
| Volume Number | 2014 |
| e-ISSN | 15596095 |
| Journal | Cold Spring Harbor Protocols |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2014-04-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Cell Separation Methods Cerebral Cortex Cytology Immunologic Techniques Motor Neurons Physiology Staining And Labeling Animals Antitoxins Metabolism Cholera Toxin Rats Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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