NDLI: Subcapsular transplantation of tissue in the kidney.

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Subcapsular transplantation of tissue in the kidney.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Shultz, Leonard D. Goodwin, Neal Ishikawa, Fumihiko Hosur, Vishnu Lyons, Bonnie L. Greiner, Dale L.
Description	Author Affiliation: Shultz LD ( The Jackson Laboratory, Bar Harbor, Maine 04609); Goodwin N ( The Jackson Laboratory, Bar Harbor, Maine 04609); Ishikawa F ( The Laboratory for Human Disease Models, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230-0045, Japan); Hosur V ( The Jackson Laboratory, Bar Harbor, Maine 04609); Lyons BL ( The Jackson Laboratory, Bar Harbor, Maine 04609); Greiner DL ( University of Massachusetts Medical School, Worcester, Massachusetts 01605.)
Abstract	Multiple sites can be used for the engraftment of primary human cells and tissues into murine hosts. For example, leukemias are usually best engrafted intravenously, but they can also be engrafted directly into the bone marrow cavity. Some solid tumors such as colon tumors grow successfully following subcutaneous engraftment, sometimes requiring provision of a Matrigel artificial basement membrane. In certain cases (e.g., human bladder cancer and ductal breast carcinoma), the use of the autochthonous site (bladder and mammary duct, respectively) is often most efficient, whereas the tumors can grow poorly when transplanted subcutaneously or heterochthonously. Here, we present a protocol for the surgical implantation of tissues under the kidney capsule. The kidney is especially suited for the transplantation of normal as well as malignant cells and tissues. It is very accessible, and transplanted tissues are well contained under the renal capsule in a highly vascularized site. Furthermore, the retroperitoneal location of the kidney, together with its separation from other organs, is advantageous both for imaging and biopsy.
File Format	HTM / HTML
ISSN	19403402
e-ISSN	15596095
DOI	10.1101/pdb.prot078089
Journal	Cold Spring Harbor Protocols
Issue Number	7
Volume Number	2014
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2014-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Clinical Laboratory Techniques Kidney Surgery Transplantation, Heterologous Animals Bowman Capsule Mice Neoplasm Transplantation
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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