| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Crowley, Lisa C. Marfell, Brooke J. Scott, Adrian P. Boughaba, Jeanne A. Chojnowski, Grace Christensen, Melinda E. Waterhouse, Nigel J. |
| Description |
Country affiliation: Australia Author Affiliation: Crowley LC ( Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia.); Marfell BJ ( Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia.); Scott AP ( Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia.); Boughaba JA ( Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia.); Chojnowski G ( Agroparistech, Paris Cedex 05, France.); Christensen ME ( Flow Cytometry and Imaging, QIMR Berghofer Medical Research Institute, Herston, Brisbane, Queensland 4006, Australia.); Waterhouse NJ ( Apoptosis and Cytotoxicity Laboratory, Mater Research, Translational Research Institute, Woolloongabba, Brisbane, Queensland 4102, Australia.) |
| Abstract |
Many cells in the body die at specific times to facilitate healthy development or because they have become old, damaged, or infected. Defects in cells that result in their inappropriate survival or untimely death can negatively impact development or contribute to a variety of human pathologies, including cancer, AIDS, autoimmune disorders, and chronic infection. Cell death may also occur following exposure to environmental toxins or cytotoxic chemicals. Although this is often harmful, it can be beneficial in some cases, such as in the treatment of cancer. The ability to objectively measure cell death in a laboratory setting is therefore essential to understanding and investigating the causes and treatments of many human diseases and disorders. Often, it is sufficient to know the extent of cell death in a sample; however, the mechanism of death may also have implications for disease progression, treatment, and the outcomes of experimental investigations. There are a myriad of assays available for measuring the known forms of cell death, including apoptosis, necrosis, autophagy, necroptosis, anoikis, and pyroptosis. Here, we introduce a range of assays for measuring cell death in cultured cells, and we outline basic techniques for distinguishing healthy cells from apoptotic or necrotic cells-the two most common forms of cell death. We also provide personal insight into where these assays may be useful and how they may or may not be used to distinguish apoptotic cell death from other death modalities. |
| File Format |
HTM / HTML |
| ISSN |
19403402 |
| Issue Number |
12 |
| Journal |
Cold Spring Harbor Protocols |
| Volume Number |
2016 |
| e-ISSN |
15596095 |
| Language |
English |
| Publisher |
Cold Spring Harbor Laboratory Press |
| Publisher Date |
2016-12-01 |
| Publisher Place |
United States |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Clinical Laboratory Techniques |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Biochemistry, Genetics and Molecular Biology |
