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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | McIntosh, J. Richard Morphew, Mary K. Giddings, Thomas H. |
| Description | Author Affiliation: McIntosh JR ( Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347.); Morphew MK ( Laboratory for 3D Electron Microscopy, University of Colorado, Boulder, Colorado 80309-0347.); Giddings TH ( Laboratory for 3D Electron Microscopy, University of Colorado, Boulder, Colorado 80309-0347.) |
| Abstract | Electron microscopy (EM) can provide images of cells with a spatial resolution that significantly surpasses that available from light microscopy (LM), even with modern methods that give LM 'super resolution.' However, EM resolution comes with costs in time spent with sample preparation, expense of instrumentation, and concerns regarding sample preparation artifacts. It is therefore important to know the limitations of EM as well as its strengths. Here we describe the most reliable methods for the preservation of fission yeast cells currently available. We describe the properties of images obtained by transmission EM (TEM) and contrast them with images from scanning EM (SEM). We also show how one can make three-dimensional TEM images and discuss several approaches to address the problem of localizing specific proteins within cells. We give references to work by others who have pursued similar goals with different methods, and we discuss briefly the complex subject of image interpretation. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| Issue Number | 1 |
| Journal | Cold Spring Harbor Protocols |
| Volume Number | 2017 |
| e-ISSN | 15596095 |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2017-01-03 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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