NDLI: Partial Purification and Characterization of Polyphenol Oxidase Isozymes in Banana Bud.

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Effects of Okadaic Acid on Embryonic Development of the Starfish Asterina pectinifera.

Partial Purification and Characterization of Polyphenol Oxidase Isozymes in Banana Bud.

Biosynthesis and Hydrolysis of Poly(Î³-glutamic acid) from Bacillus subtilis IF03335.

Biogenesis of Protein Bodies in Embryonic Axes of Soybean Seeds (Glycine max. cv. Enrei).

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Simultaneous Measurement of the Galacturonate and Neutral Sugar Contents of Pectic Substances by an Enzymic-HPLC Method

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HPLC Analysis of Commercial Casein Phosphopeptides (CPP).

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Growth-dependent Topological Alteration of Plasmid DNA in Escherichia coli Topoisomerase Mutants.

Irones and Precursors Synthesized by Iris sibirica Tissue Culture.

Purification and Characterization of the Main Isozyme of Polyphenol Oxidase in Mung Bean (Vigna mungo) Seedlings.

Structure and Biological Activity of Plant Growth Regulators Produced by Penicillium sp. No. 31f.

Analysis of Plant Pectic Substances by Electrophoresis on Cellulose Acetate Membrane.

Effects of Dissolved Oxygen Tension on Microbial Ethylene Production in Continuous Culture.

Absolute Stereochemistry of Neovasinin, a Phytotoxin Produced by the Fungus, Neocosmospora vasinfecta.

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Structural Elucidation of Rotihibin B by Tandem Mass Spectrometry.

Gibberellin-responsive Gene Expression Taking Place with Oryzains and Î³ as Cysteine Proteinases of Rice Seeds.

Photosensitized Oxygenation Reactions of ß-Thujaplicin (Hinokitiol): Formation of a New Carbon Skeleton, Tetracyclo[7.3.2.0(2,8).0(4,14)]tetradecane.

Pyrenolide D, a New Cytotoxic Fungal Metabolite from Pyrenophora teres.

Synthesis of the Enantiomers of (Z)-5-(1-Octenyl)oxacyclopentan-2-one, a Sex Pheromone of the Cupreous Chafer Beetle, Anomala cuprea Hope.

Simple Preparation of N-Substituted 4-Arylpyrrolones.

Synthesis of the Photoaffinity-labeled Grayanotoxins for Studying the Gating Mechanism of Na Channel.

Direct Chromatographic Separation of the Enantiomers of Methyl Jasmonate and Its Derivatives.

Coriolus versicolor Laccase Catalyzes the Decarboxylation of 2-(4-Hydroxyphenyl)-glycine and 4-Hydroxymandelic Acid.

Year: 1992 Volume: 56 Issue: 6

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Year: 1992 Volume: 56 Issue: 4

Year: 1992 Volume: 56 Issue: 3

Partial Purification and Characterization of Polyphenol Oxidase Isozymes in Banana Bud.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ôba, K. Iwatsuki, N. Uritani, I. Alvarez, A. M. Garcia, V. V.
Description	Country affiliation: Japan Author Affiliation: Ôba K ( a Faculty of Domestic Science , Nagoya Women's University , 3-40, Shioji, Mizuho , Nagoya 467 , Japan.); Iwatsuki N ( a Faculty of Domestic Science , Nagoya Women's University , 3-40, Shioji, Mizuho , Nagoya 467 , Japan.); Uritani I ( b Koryo International College , Sagamine, Nissin, Aichi-gun , Aichi 470-01 , Japan.); Alvarez AM ( a Faculty of Domestic Science , Nagoya Women's University , 3-40, Shioji, Mizuho , Nagoya 467 , Japan.); Garcia VV ( a Faculty of Domestic Science , Nagoya Women's University , 3-40, Shioji, Mizuho , Nagoya 467 , Japan.)
Abstract	Polyphenol oxidase was extracted from banana buds in the presences of Triton X-100, isoascorbate, and Polyclar AT, and two isozymes I and II have been separated and partially purified by chromatographies on Butyl Toyopearl 650 and DEAE-cellulose. I and II had different mobility in polyacrylamide gel electrophoresis with optimum pHs of 6.8 and 5.5, respectively. Both enzymes showed the apparent Km values of 0.5 mM for dopamine with substrate inhibitions at its higher concentrations. I and II were inhibited competitively by NaCI with the Ki values of 140 mM and 40 mM, respectively. I and II have a high heat stability, and 88 and 95% of the initial activities were retained after 1-hr incubation at 70°C, respectively.
File Format	HTM / HTML
ISSN	09168451
e-ISSN	13476947
Journal	Bioscience, Biotechnology, and Biochemistry
Issue Number	7
Volume Number	56
Language	English
Publisher	Taylor & Francis
Publisher Date	1992-01-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology

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