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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | See, Khoo Lay Elbashir, Abdalla A. Saad, Bahruddin Ali, Abdussalam Salhin Mohamed Aboul-Enein, Hassan Y. |
| Description | Country affiliation: Malaysia Author Affiliation: See KL ( School of Chemical Sciences, Universiti Sains Malaysia, Penang, Malaysia.) |
| Abstract | A simple, rapid and validated capillary electrophoretic method has been developed for the separation and determination of ofloxacin and ornidazole in pharmaceutical formulations with detection at 230 nm. Optimal conditions for the quantitative separations were investigated. Analysis times shorter than 4 min were obtained using a background electrolyte solution consisting of 25 mmol/L phosphoric acid adjusted with 1 M Tris buffer to pH 8.5, with hydrodynamic injection of 5 s and 20 kV separation voltage. The validation criteria for accuracy, precision, linearity and limits of detection and quantitation were examined and discussed. An excellent linearity was obtained in concentration range 25-250 microg/mL. The detection limits for ofloxacin and ornidazole were 1.03 +/- 0.11 and 1.80 +/- 0.06 microg/mL, respectively. The proposed method has been applied for the analysis of ofloxacin and ornidazole both individually and in a combined dosage tablet formulation. The proposed validated method showed recoveries between 96.16 and 105.23% of the nominal contents. |
| File Format | HTM / HTML |
| ISSN | 02693879 |
| Issue Number | 12 |
| Volume Number | 23 |
| e-ISSN | 10990801 |
| Journal | Biomedical Chromatography |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2009-12-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Discipline Molecular Biology Discipline Biochemistry Discipline Pharmacology Anti-bacterial Agents Analysis Electrophoresis, Capillary Methods Ofloxacin Ornidazole Pharmaceutical Preparations Chemistry Buffers Hydrogen-ion Concentration Reference Standards Reproducibility Of Results Spectrophotometry, Ultraviolet Journal Article Research Support, Non-u.s. Gov't Validation Studies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Analytical Chemistry Drug Discovery Clinical Biochemistry Biochemistry Molecular Biology Pharmacology |
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