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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Qin, Xiao-ling Bi, Hui-chang Wang, Chang-xi Li, Jia-li Wang, Xue-ding Liu, Long-shan Chen, Xiao Huang, Min |
| Description | Country affiliation: China Author Affiliation: Qin XL ( Institute of Clinical Pharmacology, School of Pharmaceutical Sciences, Sun Yat-sen University, 74 Zhongshan Road II, Guangzhou, China.) |
| Abstract | A liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was developed and validated for determining tacrolimus (FK506) in rat tissues to study the effect of Schisandra sphenanthera extract on FK506 tissue distribution. After a liquid–liquid extraction with ethyl acetate, FK506 and ascomycin (IS) were subjected to LC-MS/MS analysis using positive electrospray ionization under multiple reactions monitoring mode. Chromatographic separation of FK506 and ascomycin was achieved on a Hypersil BDS $C_{18}$ column with a mobile phase consisting of methanol-water (containing 2 mM ammonium acetate, 95 : 5, v/v). The intra- and inter-batch precision of the method were less than 8.8 and 9.8%, respectively. The intra- and inter-batch accuracies ranged from 97.5 to 104.0%. The lowest limit of quantification for FK506 was 0.5 ng/mL. The method was applied to a FK506 tissue distribution study with or without a dose of Wuzhi (WZ) tablet. Most of the FK506 tissue concentrations were slightly increased after a concomitant WZ tablet dose, but the whole blood concentration of FK506 was dramatically increased 3-fold after a concomitant WZ tablet dose. These results indicated that the LC-MS/MS method was rapid and sensitive enough to quantify FK506 in different rat tissues, and strict drug monitoring is recommended when co-administering WZ tablet in clinical use. Copyright © 2009 John Wiley & Sons, Ltd. |
| File Format | HTM / HTML |
| ISSN | 02693879 |
| Issue Number | 4 |
| Volume Number | 24 |
| e-ISSN | 10990801 |
| Journal | Biomedical Chromatography |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2010-04-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Discipline Molecular Biology Discipline Biochemistry Discipline Pharmacology Chromatography, High Pressure Liquid Methods Plant Extracts Pharmacology Schisandra Chemistry Tacrolimus Pharmacokinetics Tandem Mass Spectrometry Animals Drug Interactions Linear Models Male Rats Rats, Sprague-dawley Reproducibility Of Results Sensitivity And Specificity Analogs & Derivatives Analysis Tissue Distribution Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Analytical Chemistry Drug Discovery Clinical Biochemistry Biochemistry Molecular Biology Pharmacology |
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