| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Sinha, Amit Kumar Kapotwe, Mumba Dabi, Shambel Boki Montes, Caroline da Silva Shrivastava, Jyotsna Blust, Ronny De Boeck, Gudrun |
| Description |
Author Affiliation: Sinha AK ( Systemic Physiological and Ecotoxicological Research, Department of Biology, University of Antwerp, Groenenborgerlaan 171, BE-2020 Antwerp, Belgium. Electronic address: sinha_cife@rediffmail.com.); Kapotwe M ( Systemic Physiological and Ecotoxicological Research, Department of Biology, University of Antwerp, Groenenborgerlaan 171, BE-2020 Antwerp, Belgium.); Dabi SB ( Systemic Physiological and Ecotoxicological Research, Department of Biology, University of Antwerp, Groenenborgerlaan 171, BE-2020 Antwerp, Belgium.); Montes Cda S ( Systemic Physiological and Ecotoxicological Research, Department of Biology, University of Antwerp, Groenenborgerlaan 171, BE-2020 Antwerp, Belgium); Shrivastava J ( Systemic Physiological and Ecotoxicological Research, Department of Biology, University of Antwerp, Groenenborgerlaan 171, BE-2020 Antwerp, Belgium.); Blust R ( Systemic Physiological and Ecotoxicological Research, Department of Biology, University of Antwerp, Groenenborgerlaan 171, BE-2020 Antwerp, Belgium.); De Boeck G ( Systemic Physiological and Ecotoxicological Research, Department of Biology, University of Antwerp, Groenenborgerlaan 171, BE-2020 Antwerp, Belgium.) |
| Abstract |
The main objective of this study was to understand the mode of interaction between waterborne copper (Cu) and high environmental ammonia (HEA) exposure on freshwater fish, and how they influence the toxicity of each other when present together. For this purpose, individual and combined effects of Cu and HEA were examined on selected physiological and ion-regulatory processes and changes at transcript level in the common carp (Cyprinus carpio). Juvenile carp were exposed to 2.6µM Cu (25% of the 96h LC50value) and to 0.65mM ammonia (25% of the 96h LC50value) singly and as a mixture for 12h, 24h, 48h, 84h and 180h. Responses such as ammonia (Jamm) and urea (Jurea) excretion rate, plasma ammonia and urea, plasma ions (Na(+), Cl(-) and K(+)), muscle water content (MWC) as well as branchial Na(+)/K(+)-ATPase (NKA) and H(+)-ATPase activity, and branchial mRNA expression of NKA, H(+)-ATPase, Na(+)/H(+) exchanger (NHE-3) and Rhesus (Rh) glycoproteins were investigated under experimental conditions. Results show that Jamm was inhibited during Cu exposure, while HEA exposed fish were able to increase excretion efficiently. In the combined exposure, Jamm remained at the control levels indicating that Cu and HEA abolished each other's effect. Expression of Rhcg (Rhcg-a and Rhcg-b) mRNA was upregulated during HEA, thereby facilitated ammonia efflux out of gills. On the contrary, Rhcg-a transcript level declined following Cu exposure which might account for Cu induced Jamm inhibition. Likewise, Rhcg-a was also down-regulated in Cu-HEA co-exposed fish whilst a temporary increment was noted for Rhch-b. Fish exposed to HEA displayed pronounced up-regulation in NKA expression and activity and stable plasma ion levels. In both the Cu exposure alone and combined Cu-HEA exposure, ion-osmo homeostasis was adversely affected, exemplified by the significant reduction in plasma [Na(+)] and [Cl(-)], and elevated plasma [K(+)], along with an elevation in MWC. These changes were accompanied by a decline in NKA activity. Gill H(+)-ATPase mRNA levels and activities were not affected by either Cu or HEA or both. Likewise, NHE-3 expression remained unaltered but tended to be numerically higher during HEA exposure. Overall, these data suggest that at equitoxic concentrations (25% of 96h LC50), the individual effect of Cu is more harmful while HEA induces quicker adaptive responses. Our findings also denote a competitive mode of interaction, exemplified by the inhibition of HEA -mediated adaptive responses in the presence of Cu. |
| File Format |
HTM / HTML |
| ISSN |
0166445X |
| Volume Number |
170 |
| e-ISSN |
18791514 |
| Journal |
Aquatic Toxicology |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2016-01-01 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Toxicology Ammonia Toxicity Carps Metabolism Copper Gills Drug Effects Water Pollutants, Chemical Blood Animals Blood Proteins Growth & Development Environmental Exposure Ions Chemistry Muscle, Skeletal Rna, Messenger Sodium Sodium-potassium-exchanging Atpase Genetics Up-regulation Urea Vacuolar Proton-translocating Atpases Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Health, Toxicology and Mutagenesis Aquatic Science |
