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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Vassylyeva, Marina N. Svetlov, Vladimir Klyuyev, Sergiy Devedjiev, Yancho D. Artsimovitch, Irina Vassylyev, Dmitry G. |
| Description | Country affiliation: United States Author Affiliation: Vassylyeva MN ( Department of Biochemistry and Molecular Genetics, University of Alabama at Birmingham, School of Medicine, 402B Kaul Genetics Building, 720 20th Street South, Birmingham, AL 35294, USA.) |
| Abstract | The bacterial transcriptional factor and virulence regulator RfaH binds to rapidly moving transcription elongation complexes through specific interactions with the exposed segment of the non-template DNA strand. To elucidate this unusual mechanism of recruitment, determination of the three-dimensional structure of RfaH and its complex with DNA was initiated. To this end, the Escherichia coli rfaH gene was cloned and expressed. The purified protein was crystallized by the sitting-drop vapor-diffusion technique. The space group was P6(1)22 or P6(5)22, with unit-cell parameters a = b = 45.46, c = 599.93 A. A complex of RfaH and a nine-nucleotide oligodeoxyribonucleotide was crystallized by the same technique, but under different crystallization conditions, yielding crystals that belonged to space group P1 (unit-cell parameters a = 36.79, b = 44.01, c = 62.37 A, alpha = 80.62, beta = 75.37, gamma = 75.41 degrees ). Complete diffraction data sets were collected for RfaH and its complex with DNA at 2.4 and 1.6 A resolution, respectively. Crystals of selenomethionine-labeled proteins in both crystal forms were obtained by cross-microseeding using the native microcrystals. The structure determination of RfaH and its complex with DNA is in progress. |
| File Format | HTM / HTML |
| ISSN | 2053230X |
| e-ISSN | 17443091 |
| Journal | Acta Crystallographica Section F Structural Biology and Crystallization Communications |
| Issue Number | Pt 10 |
| Volume Number | 62 |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2006-10-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Discipline Crystallography Discipline Biophysics Discipline Molecular Biology Discipline Biochemistry Chemistry Escherichia Coli Proteins Peptide Elongation Factors Trans-activators Cloning, Molecular Crystallization Crystallography, X-ray Metabolism Escherichia Coli Genetics Operon Transcription, Genetic Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Structural Biology Medicine Biochemistry Biophysics Condensed Matter Physics |
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