NDLI: The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein.

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The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein.

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The use of Co2+ for crystallization and structure determination, using a conventional monochromatic X-ray source, of flax rust avirulence protein.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Guncar, Gregor Wang, Ching-I A. Forwood, Jade K. Teh, Trazel Catanzariti, Ann-Maree Ellis, Jeffrey G. Dodds, Peter N. Kobe, Bostjan
Description	Country affiliation: Australia Author Affiliation: Guncar G ( School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Qld 4072, Australia.)
Abstract	Metal-binding sites are ubiquitous in proteins and can be readily utilized for phasing. It is shown that a protein crystal structure can be solved using single-wavelength anomalous diffraction based on the anomalous signal of a cobalt ion measured on a conventional monochromatic X-ray source. The unique absorption edge of cobalt (1.61 A) is compatible with the Cu K alpha wavelength (1.54 A) commonly available in macromolecular crystallography laboratories. This approach was applied to the determination of the structure of Melampsora lini avirulence protein AvrL567-A, a protein with a novel fold from the fungal pathogen flax rust that induces plant disease resistance in flax plants. This approach using cobalt ions may be applicable to all cobalt-binding proteins and may be advantageous when synchrotron radiation is not readily available.
File Format	HTM / HTML
ISSN	2053230X
e-ISSN	17443091
Journal	Acta Crystallographica Section F Structural Biology and Crystallization Communications
Issue Number	Pt 3
Volume Number	63
Language	English
Publisher	Wiley
Publisher Date	2007-03-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Crystallography Discipline Biophysics Discipline Molecular Biology Discipline Biochemistry Basidiomycota Chemistry Cobalt Crystallography, X-ray Flax Microbiology Fungal Proteins Pathogenicity Crystallization Plant Diseases Virulence Physiology Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Structural Biology Medicine Biochemistry Biophysics Condensed Matter Physics

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