NDLI: Purification and crystallization of Vibrio fischeri CcdB and its complexes with fragments of gyrase and CcdA.

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Purification and crystallization of Vibrio fischeri CcdB and its complexes with fragments of gyrase and CcdA.

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Purification and crystallization of Vibrio fischeri CcdB and its complexes with fragments of gyrase and CcdA.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	De Jonge, Natalie Buts, Lieven Vangelooven, Joris Mine, Natacha Van Melderen, Laurence Wyns, Lode Loris, Remy
Description	Country affiliation: Belgium Author Affiliation: De Jonge N ( Department of Molecular and Cellular Interactions, VIB, Pleinlaan 2, 1050 Brussels, Belgium. ndejonge@vub.ac.be)
Abstract	The ccd toxin-antitoxin module from the Escherichia coli F plasmid has a homologue on the Vibrio fischeri integron. The homologue of the toxin (CcdB(Vfi)) was crystallized in two different crystal forms. The first form belongs to space group I23 or I2(1)3, with unit-cell parameter a = 84.5 A, and diffracts to 1.5 A resolution. The second crystal form belongs to space group C2, with unit-cell parameters a = 58.5, b = 43.6, c = 37.5 A, beta = 110.0 degrees, and diffracts to 1.7 A resolution. The complex of CcdB(Vfi) with the GyrA14(Vfi) fragment of V. fischeri gyrase crystallizes in space group P2(1)2(1)2(1), with unit-cell parameters a = 53.5, b = 94.6, c = 58.1 A, and diffracts to 2.2 A resolution. The corresponding mixed complex with E. coli GyrA14(Ec) crystallizes in space group C2, with unit-cell parameters a = 130.1, b = 90.8, c = 58.1 A, beta = 102.6 degrees, and diffracts to 1.95 A. Finally, a complex between CcdB(Vfi) and part of the F-plasmid antitoxin CcdA(F) crystallizes in space group P2(1)2(1)2(1), with unit-cell parameters a = 46.9, b = 62.6, c = 82.0 A, and diffracts to 1.9 A resolution.
File Format	HTM / HTML
ISSN	2053230X
e-ISSN	17443091
Journal	Acta Crystallographica Section F Structural Biology and Crystallization Communications
Issue Number	Pt 4
Volume Number	63
Language	English
Publisher	Wiley
Publisher Date	2007-04-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Crystallography Discipline Biophysics Discipline Molecular Biology Discipline Biochemistry Aliivibrio Fischeri Chemistry Bacterial Proteins Isolation & Purification Dna Gyrase Enzymology Amino Acid Sequence Crystallization Dna Primers Molecular Sequence Data Protein Conformation Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Structural Biology Medicine Biochemistry Biophysics Condensed Matter Physics

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