NDLI: Crystallization and preliminary X-ray analysis of Atg10.

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Crystallization and preliminary X-ray analysis of Atg10.

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Crystallization and preliminary X-ray analysis of Atg10.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yamaguti, Masaya Suzuki, Nobuo N. Fujioka, Yuko Ohsumi, Yoshinori Inagaki, Fuyuhiko
Description	Country affiliation: Japan Author Affiliation: Yamaguti M ( Department of Structural Biology, Graduate School of Pharmaceutical Sciences, Hokkaido University, N-12, W-6, Kita-ku, Sapporo 060-0812, Japan.)
Abstract	Atg10 is an E2-like enzyme that catalyzes the conjugation reaction between Atg12 and Atg5. The Atg12-Atg5 conjugate is essential for autophagy, the bulk degradation process of cytoplasmic components by the vacuolar/lysosomal system. Microcrystals of Saccharomyces cerevisiae Atg10 were obtained by the free-interface diffusion method using polyethylene glycol and sodium acetate as precipitants. Using these precipitants, large crystals suitable for data collection were obtained using the sitting-drop vapour-diffusion method. The crystals belong to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 51.61, c = 256.16 A, and are estimated to contain two protein molecules per asymmetric unit. A native data set was collected to 2.3 A resolution from a single crystal.
File Format	HTM / HTML
ISSN	2053230X
e-ISSN	17443091
Journal	Acta Crystallographica Section F Structural Biology and Crystallization Communications
Issue Number	Pt 5
Volume Number	63
Language	English
Publisher	Wiley
Publisher Date	2007-05-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Crystallography Discipline Biophysics Discipline Molecular Biology Discipline Biochemistry Saccharomyces Cerevisiae Proteins Chemistry Crystallization Crystallography, X-ray Protein Conformation Isolation & Purification Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Structural Biology Medicine Biochemistry Biophysics Condensed Matter Physics

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