NDLI: Purification, crystallization and preliminary X-ray crystallographic analysis of branched-chain aminotransferase from Deinococcus radiodurans.

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Purification, crystallization and preliminary X-ray crystallographic analysis of branched-chain aminotransferase from Deinococcus radiodurans.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, Chung-Der Huang, Tien-Feng Lin, Chih-Hao Guan, Hong-Hsiang Hsieh, Yin-Cheng Lin, Yi-Hung Huang, Yen-Chieh Liu, Ming-Yih Chang, Wen-Chang Chen, Chun-Jung
Description	Country affiliation: Taiwan Author Affiliation: Chen CD ( Department of Physics, National Tsing-Hua University, Hsinchu 30013, Taiwan.)
Abstract	The branched-chain amino-acid aminotransferase (BCAT), which requires pyridoxal 5'-phosphate (PLP) as a cofactor, is a key enzyme in the biosynthetic pathway of the hydrophobic amino acids leucine, isoleucine and valine. DrBCAT from Deinococcus radiodurans, which has a molecular weight of 40.9 kDa, was crystallized using the hanging-drop vapour-diffusion method. According to X-ray diffraction data to 2.50 A resolution from a DrBCAT crystal, the crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 56.37, b = 90.70, c = 155.47 A. Preliminary analysis indicates the presence of two DrBCAT molecules in the asymmetric unit, with a solvent content of 47.52%.
File Format	HTM / HTML
ISSN	2053230X
e-ISSN	17443091
Journal	Acta Crystallographica Section F Structural Biology and Crystallization Communications
Issue Number	Pt 6
Volume Number	63
Language	English
Publisher	Wiley
Publisher Date	2007-06-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Crystallography Discipline Biophysics Discipline Molecular Biology Discipline Biochemistry Bacterial Proteins Isolation & Purification Deinococcus Enzymology Transaminases Chemistry Crystallization Crystallography, X-ray Isoleucine Leucine Valine Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Structural Biology Medicine Biochemistry Biophysics Condensed Matter Physics

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