NDLI: Expression, purification, crystallization and preliminary X-ray analysis of the polysaccharide lyase RB5312 from the marine planctomycete Rhodopirellula baltica.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Dabin, Jérôme Jam, Murielle Czjzek, Mirjam Michel, Gurvan
Description	Country affiliation: France Author Affiliation: Dabin J ( UPMC University Paris 06, UMR 7139, Marine Plants and Biomolecules, Station Biologique de Roscoff, F-29682 Roscoff, Bretagne, France.)
Abstract	Polysaccharide lyases belonging to family PL1 act on pectins. These anionic polymers are usually produced by terrestrial plants and therefore pectinolytic enzymes are not frequently observed in marine microorganisms. The protein RB5312 from the marine bacterium Rhodopirellula baltica is distantly related to family PL1 pectate lyases, but its exact function is unclear. In this study, the expression and purification of a recombinant form of RB5312 are described. This protein was crystallized using the hanging-drop vapour-diffusion method. The crystals belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 39.05, b = 144.05, c = 153.97 A, alpha = beta = gamma = 90 degrees. A complete data set was collected to 1.8 A resolution from a native crystal.
File Format	HTM / HTML
ISSN	2053230X
e-ISSN	17443091
DOI	10.1107/S1744309108004387
Journal	Acta Crystallographica Section F Structural Biology and Crystallization Communications
Issue Number	Pt 3
Volume Number	64
Language	English
Publisher	Wiley
Publisher Date	2008-03-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Crystallography Discipline Biophysics Discipline Molecular Biology Discipline Biochemistry Bacteria Enzymology Gene Expression Polysaccharide-lyases Chemistry Isolation & Purification Genetics Cloning, Molecular Crystallization Oceans And Seas Metabolism X-ray Diffraction Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Structural Biology Medicine Biochemistry Biophysics Condensed Matter Physics

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