NDLI: Crystallization and preliminary X-ray diffraction analysis of the N-terminal domain of Mrs2, a magnesium ion transporter from yeast inner mitochondrial membrane

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Khan, Muhammad Bashir Sjöblom, Björn Schweyen, Rudolf J. Djinovic-Carugo, Kristina
Description	Country affiliation: Austria Author Affiliation: Khan MB ( Department for Structural and Computational Biology, Max F. Perutz Laboratories, University of Vienna, Vienna, Austria.)
Abstract	Mrs2 transporters are distantly related to the major bacterial Mg(2+) transporter CorA and to Alr1, which is found in the plasma membranes of lower eukaryotes. Common features of all Mrs2 proteins are the presence of an N-terminal soluble domain followed by two adjacent transmembrane helices (TM1 and TM2) near the C-terminus and of the highly conserved F/Y-G-M-N sequence motif at the end of TM1. The inner mitochondrial domain of the Mrs2 from Saccharomyces cerevisae was overexpressed, purified and crystallized in two different crystal forms corresponding to an orthorhombic and a hexagonal space group. The crystals diffracted X-rays to 1.83 and 4.16 A resolution, respectively. Matthews volume calculations suggested the presence of one molecule per asymmetric unit in the orthorhombic crystal form and of five or six molecules per asymmetric unit in the hexagonal crystal form. The phase problem was solved for the orthorhombic form by a single-wavelength anomalous dispersion experiment exploiting the sulfur anomalous signal.
File Format	HTM / HTML
ISSN	2053230X
e-ISSN	17443091
DOI	10.1107/S1744309110012212
Journal	Acta Crystallographica Section F Structural Biology and Crystallization Communications
Issue Number	Pt 6
Volume Number	66
Language	English
Publisher	Wiley
Publisher Date	2010-06-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Crystallography Discipline Biophysics Discipline Molecular Biology Discipline Biochemistry Cation Transport Proteins Chemistry Ion Channels Mitochondrial Membranes Mitochondrial Proteins Saccharomyces Cerevisiae Proteins Saccharomyces Cerevisiae Crystallization Crystallography, X-ray Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Structural Biology Medicine Biochemistry Biophysics Condensed Matter Physics

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