NDLI: Expression, purification, crystallization and preliminary crystallographic analysis of PA3885 (TpbA) from Pseudomonas aeruginosa PAO1.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yang, Wen Li, Kan Bai, Yuwei Zhou, Ruimin Zhou, Weihong Bartlam, Mark
Description	Author Affiliation: Yang W ( Tianjin Key Laboratory of Protein Science, College of Life Sciences, Nankai University, Tianjin 300071, People's Republic of China.)
Abstract	Biofilms are important in cell communication and growth in most bacteria and are also responsible for most human clinical infections and diseases. Quorum-sensing systems have been identified to be crucial for biofilm formation and regulation. PA3885 (TpbA), a tyrosine phosphatase, is reported to convert extracellular quorum-sensing signals into internal gene-cascade reactions that result in reduced biofilm formation in the opportunistic pathogen Pseudomonas aeruginosa. Here, PA3885 from P. aeruginosa PAO1 was expressed, purified and crystallized. Single crystals were studied by X-ray crystallography and native diffraction data were collected to 2.8âÅ resolution. These crystals were determined to belong to space group C2. It was not possible to conclusively determine the number of proteins in the asymmetric unit from the preliminary X-ray diffraction data analysis alone and attempts to determine the crystal structure of PA3885 are currently under way.
File Format	HTM / HTML
ISSN	2053230X
e-ISSN	17443091
DOI	10.1107/S1744309110034317
Journal	Acta Crystallographica Section F Structural Biology and Crystallization Communications
Issue Number	Pt 11
Volume Number	66
Language	English
Publisher	Wiley
Publisher Date	2010-11-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Crystallography Discipline Biophysics Discipline Molecular Biology Discipline Biochemistry Protein Tyrosine Phosphatases Chemistry Pseudomonas Aeruginosa Enzymology Crystallization Crystallography, X-ray Gene Expression Genetics Isolation & Purification Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Structural Biology Medicine Biochemistry Biophysics Condensed Matter Physics

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