NDLI: Mechanotransduction of trigeminal ganglion neurons innervating inner walls of rat anterior eye chambers.

Please wait, while we are loading the content...

Histamine activates p38 MAP kinase and alters local lamellipodia dynamics, reducing endothelial barrier integrity and eliciting central movement of actin fibers

Tumor necrosis factor- induces a biphasic change in claudin-2 expression in tubular epithelial cells: role in barrier functions.

Pyk and ERK your way to the hub by taking a RSK 2. Focus on 'Regulation of NHE3 by lysophosphatidic acid is mediated by phosphorylation of NHE3 by RSK2'.

Year: 2015 Volume: 308

Year: 2014 Volume: 307

Year: 2014 Volume: 306

Year: 2013 Volume: 305

Year: 2013 Volume: 304

Year: 2012 Volume: 303

Year: 2012 Volume: 302

Year: 2011 Volume: 301

Year: 2011 Volume: 300

Year: 2010 Volume: 299

Year: 2010 Volume: 298

Year: 2009 Volume: 297

Year: 2009 Volume: 296

Year: 2008 Volume: 295

Year: 2008 Volume: 294

Year: 2007 Volume: 293

Year: 2007 Volume: 292

Year: 2006 Volume: 291

Year: 2006 Volume: 290

Year: 2005 Volume: 289

Year: 2005 Volume: 288

Year: 2004 Volume: 286

Year: 2003 Volume: 284

Year: 2002 Volume: 283

Year: 2002 Volume: 282

Year: 2001 Volume: 281

Year: 2001 Volume: 280

Year: 2000 Volume: 279

Mechanotransduction of trigeminal ganglion neurons innervating inner walls of rat anterior eye chambers.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Meng, Qingli Fang, Peng Hu, Zhuangli Ling, Yun Liu, Haixia
Description	Author Affiliation: Meng Q ( Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China); Fang P ( Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China.); Hu Z ( Department of Pharmacology, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China.); Ling Y ( Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China); Liu H ( Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, China)
Abstract	To address mechanoreceptive roles of trigeminal ganglion (TG) nerve endings in the inner walls of rat anterior eye chambers, we investigated the mechanotransduction process and mechanosensitive (MS) channel on somata of TG neurons innervating this area in vitro. Rat TG neurons innervating inner walls of anterior chambers were labeled by anterior chamber injection of 1,1'-dilinoleyl-3,3,3',3'-tetramethylindocarbocyanine, 4-chlorobenzenesulfonate (FAST DiI). The neuronal cell bodies were voltage clamped using a whole cell patch-clamp technique, while it was deformed by ejection of bath solution to verify mechanotransduction. Immunofluorescence staining was performed on sections of TG ganglia to determine the specific MS channel proteins. Mechanical stimuli induced MS currents in 55 out of 96 FAST DiI-labeled TG neurons. The MS currents exhibited mechanical intensity-dependent and clamp voltage-dependent characteristics. Mechanical stimulation further enhanced the membrane potential and increased the frequency of action potentials. Transient receptor potential ankyrin 1 (TRPA1), TRP vanilloid 4 (TRPV4), acid-sensing ion channel (ASIC) 2 and ASIC3 channel proteins were expressed in FAST DiI-labeled TG neurons. The inhibitory effect of HC-030031, a specific inhibitor of TRPA1, on MS currents demonstrated that TRPA1 was an essential MS channel protein. Taken together, our results show that mechanical stimuli induce MS currents via MS channels such as TRPA1 to trigger mechanotransduction in TG neurons innervating inner walls of anterior chambers. Our results indicate the existence of mechanoreceptive TG nerve endings in inner walls of anterior chambers. Whether the mechanoreceptive TG nerve endings play a role in intraocular pressure sensation warrants further investigation.
File Format	HTM / HTML
ISSN	03636143
e-ISSN	15221563
Journal	American Journal of Physiology - Cell Physiology
Issue Number	1
Volume Number	309
Language	English
Publisher	American Physiological Society
Publisher Date	2015-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Cell Biology Anterior Chamber Mechanoreceptors Metabolism Mechanotransduction, Cellular Trigeminal Ganglion Acid Sensing Ion Channels Action Potentials Animals Cells, Cultured Fluorescent Antibody Technique Neuroanatomical Tract-tracing Techniques Patch-clamp Techniques Physical Stimulation Pressure Presynaptic Terminals Rats, Sprague-dawley Trpc Cation Channels Trpv Cation Channels Cytology Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Physiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Bimatoprost Increases Mechanosensitivity of Trigeminal Ganglion Neurons Innervating the Inner Walls of Rat Anterior Chambers via Activation of TRPA1.

Cannabinoid WIN 55,212-2 regulates TRPV1 phosphorylation in sensory neurons.

TRPA1-mediated responses in trigeminal sensory neurons: interaction between TRPA1 and TRPV1

Electrophysiological Mechanisms and Validation of Ferritin-Based Magnetogenetics for Remote Control of Neurons.

Functional TRP and ASIC-like channels in cultured urothelial cells from the rat

Role of calcium ions in the positive interaction between TRPA1 and TRPV1 channels in bronchopulmonary sensory neurons

Transient receptor potential A1 mediates an osmotically activated ion channel.

Ultrastructural analysis of the synaptic connectivity of TRPV1-expressing primary afferent terminals in the rat trigeminal caudal nucleus.

Primary afferents with TRPM8 and TRPA1 profiles target distinct subpopulations of rat superficial dorsal horn neurones.

Mechanotransduction of trigeminal ganglion neurons innervating inner walls of rat anterior eye chambers.

Similar Documents

Bimatoprost Increases Mechanosensitivity of Trigeminal Ganglion Neurons Innervating the Inner Walls of Rat Anterior Chambers via Activation of TRPA1.

Cannabinoid WIN 55,212-2 regulates TRPV1 phosphorylation in sensory neurons.

TRPA1-mediated responses in trigeminal sensory neurons: interaction between TRPA1 and TRPV1

Electrophysiological Mechanisms and Validation of Ferritin-Based Magnetogenetics for Remote Control of Neurons.

Functional TRP and ASIC-like channels in cultured urothelial cells from the rat

Role of calcium ions in the positive interaction between TRPA1 and TRPV1 channels in bronchopulmonary sensory neurons

Transient receptor potential A1 mediates an osmotically activated ion channel.

Ultrastructural analysis of the synaptic connectivity of TRPV1-expressing primary afferent terminals in the rat trigeminal caudal nucleus.

Primary afferents with TRPM8 and TRPA1 profiles target distinct subpopulations of rat superficial dorsal horn neurones.

Mechanotransduction of trigeminal ganglion neurons innervating inner walls of rat anterior eye chambers.