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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Favaro, Marco Sarti, Mario Fontana, Carla |
| Description | Country affiliation: Italy Author Affiliation: Favaro M ( Department of Experimental Medicine and Surgery, 'Tor Vergata' University of Rome, Via Montpellier 1, 00133, Rome, Italy.) |
| Abstract | The spread of multi-resistant enterobacteria, particularly carbapenem-resistant Enterobacteriaceae (CRE), in both community and hospital settings is a global problem. The phenotypic identification of CRE is complex, occasionally inconclusive and time consuming. However, commercially available molecular assays are very expensive, and many do not allow the simultaneous identification of all genetic markers of resistance that have been recognised in CRE (bla KPC, bla OXA-48, bla VIM and bla NDM). The aim of the present study is to describe a new test: a multiplex real time PCR probe-based assay designed for the simultaneous detection of KPC, OXA-48, VIM and NDM in a short time (no longer than 90 min from the extraction of DNA to detection). Our assay correctly identified 63 CRE isolates and all standard reference strains tested, in agreement with and extending the results of phenotypic identification tests; additionally, a KPC-VIM co-expressing Enterobacter aerogenes isolate was identified using the new assay, whereas traditional methods failed to detect it. The assay was also able to correctly detect 28 CRE-producers from 50 positive blood cultures, again detecting, in four specimens, the presence of CRE co-expressing KPC and VIM, which were only partially identified by traditional methods. Finally, when used directly on rectal swabs, the assay enabled the identification of CRE-carrier patients, for whom isolation is mandatory in a hospital setting. |
| File Format | HTM / HTML |
| ISSN | 09593993 |
| Issue Number | 11 |
| Volume Number | 30 |
| e-ISSN | 15730972 |
| Journal | World Journal of Microbiology and Biotechnology |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2014-11-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Oligonucleotide Probes Discipline Microbiology Microbiology Sensitivity And Specificity Isolation & Purification Real-time Polymerase Chain Reaction Metabolism Multiplex Polymerase Chain Reaction Journal Article Discipline Biotechnology Bacterial Proteins Enterobacteriaceae Infections Time Factors Beta-lactamases Classification Genetics Diagnosis Enzymology Evaluation Studies Methods Enterobacteriaceae |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Medicine Applied Microbiology and Biotechnology Biotechnology |
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