NDLI: Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina.

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Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Rinland, María Emilia Gómez, Marisa Anahí
Spatial Coverage	Argentina
Description	Author Affiliation: Rinland ME ( Department of Agronomy, Centro de Recursos Renovables de la Zona Semiárida (CERZOS-CONICET), Universidad Nacional del Sur, San Andrés 800, 8000, Bahía Blanca, Argentina, merinland@cerzos-conicet.gob.ar.)
Abstract	Onion production in Argentina generates a significant amount of waste. Finding an effective method to recycle it is a matter of environmental concern. Among organic waste reuse techniques, anaerobic digestion could be a valuable alternative to current practices. Substrate inoculation with appropriate bacterial strains enhances the rate-limiting step (hydrolysis) of anaerobic digestion of biomass wastes. Selection of indigenous bacteria with the ability to degrade onion waste could be a good approach to find a suitable bioaugmentation or pretreatment agent. We isolated bacterial strains from onion waste in different degradation stages and from different localities. In order to characterize and select the best candidates, we analyzed the growth patterns of the isolates in a medium prepared with onion juice as the main source of nutrients and we evaluated carbon source utilization. Nine strains were selected to test their ability to grow using onion tissue and the five most remarkable ones were identified by 16S rRNA gene sequencing. Strains belonged to the genera Pseudoxanthomonas, Bacillus, Micrococcus and Pseudomonas. Two strains, Bacillus subtilis subsp. subtillis MB2-62 and Pseudomonas poae VE-74 have characteristics that make them promising candidates for bioaugmentation or pretreatment purposes.
File Format	HTM / HTML
ISSN	09593993
Issue Number	3
Volume Number	31
e-ISSN	15730972
Journal	World Journal of Microbiology and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2015-03-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Research Support, Non-u.s. Gov't Discipline Microbiology Argentina Onions Rna, Ribosomal, 16s Isolation & Purification Molecular Sequence Data Metabolism Carbon Industrial Waste Sequence Analysis, Dna Journal Article Discipline Biotechnology Growth & Development Chemistry Dna, Ribosomal Culture Media Dna, Bacterial Classification Bacteria Genetics Medical Waste Disposal Methods
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

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Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina

Isolation and characterization of alginate-degrading bacteria for disposal of seaweed wastes.

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Isolation and characterization of onion degrading bacteria from onion waste produced in South Buenos Aires province, Argentina.