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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Schillaci, Domenico Spinello, Angelo Cusimano, Maria Grazia Cascioferro, Stella Barone, Giampaolo Vitale, Maria Arizza, Vincenzo |
| Description | Country affiliation: Italy Author Affiliation: Schillaci D ( Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Via Archirafi, 32, 90123, Palermo, Italy. domenico.schillaci@unipa.it.); Spinello A ( Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Via Archirafi, 32, 90123, Palermo, Italy.); Cusimano MG ( IEMEST, Istituto Euromediterraneo di Scienza e Tecnologia, Via Michele Miraglia, 20, 90139, Palermo, Italy.); Cascioferro S ( Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Via Archirafi, 32, 90123, Palermo, Italy.); Barone G ( Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Via Archirafi, 32, 90123, Palermo, Italy.); Vitale M ( IEMEST, Istituto Euromediterraneo di Scienza e Tecnologia, Via Michele Miraglia, 20, 90139, Palermo, Italy.); Arizza V ( Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche, Via Archirafi, 32, 90123, Palermo, Italy.) |
| Abstract | Conventional antibiotics might fail in the treatment of biofilm-associated infections causing infection recurrence and chronicity. The search for antimicrobial peptides has been performed with the aim to discover novel anti-infective agents active on pathogens in both planktonic and biofilm associated forms. The fragment 9-19 of human thymosin ß4 was studied through 1 µs MD simulation. Two main conformations of the peptide were detected, both constituted by a central hydrophobic core and by the presence of peripheral charged residues suggesting a possible mechanism of interaction with two models of biological membranes, related to eukaryotic or bacterial membrane respectively. In addition, the peptide was chemically synthesized and its antimicrobial activity was tested in vitro against planktonic and biofilm form of a group of reference strains of Staphylococcus spp. and one P. aeruginosa strain. The human thymosin ß4 fragment EIEKFDKSKLK showed antibacterial activity against staphylococcal strains and Pseudomonas aeruginosa ATCC 15442 at concentrations from 12.5 to 6.2 mg/ml and inhibited biofilm formation at sub-inhibitory concentrations (3.1-0.75 mg/ml). The activity of the fragment in inhibiting biofilm formation, could be due to the conformations highlighted by the MD simulations, suggesting its interaction with the bacterial membrane. Human thymosin ß4 fragment can be considered a promising lead compound to develop novel synthetic or recombinant derivatives with improved pharmaceutical potential. |
| File Format | HTM / HTML |
| ISSN | 09593993 |
| Issue Number | 8 |
| Journal | World Journal of Microbiology and Biotechnology |
| Volume Number | 32 |
| e-ISSN | 15730972 |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2016-08-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Medicine Applied Microbiology and Biotechnology Biotechnology |
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