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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Jabeen, Khajista Javaid, Arshad Ahmad, Ejaz Athar, Makshoof |
| Description | Country affiliation: Pakistan Author Affiliation: Jabeen K ( Institute of Mycology and Plant Pathology, University of the Punjab, Quaid-e-Azam Campus, Lahore, Pakistan.) |
| Abstract | The antifungal activity of Melia azedarach L. leaves was investigated against Ascochyta rabiei (Pass.) Lab., the cause of destructive blight disease of chickpea (Cicer arietinum L.). Bioassay guided fractionation revealed that the chloroform fraction of the methanolic extract of M. azedarach leaves was highly effective against A. rabiei. Six compounds, namely ß-sitosterol (1), ß-amyrin (2), ursolic acid (3), benzoic acid (4), 3,5 dimethoxybenzoic acid (5) and maesol (6) were isolated from the chloroform fraction through column chromatography. The in vitro antifungal activity of compounds 2-5 was evaluated against A. rabiei. A commercial fungicide, mancozeb, was used as a positive control. Different concentrations of mancozeb and the isolated compounds, ranging from 0.0039 to 4 mg mL(-1), were used in the antifungal bioassay, and data regarding minimum inhibitory concentration (MIC) was recorded 24, 48 and 72 h after incubation. All concentrations of mancozeb inhibited the fungal spore germination at all three incubation periods. The tested compounds exhibited variable antifungal activity against the target fungal pathogens. All compounds showed their highest antifungal activity after 24 h of incubation. Compound 2 was found to be the most effective, with an MIC of 0.0156 mg mL(-1), followed by compounds 3, 4 and 5, with MIC values of 0.0312, 0.25 and 0.125 mg mL(-1), respectively. |
| File Format | HTM / HTML |
| ISSN | 14786419 |
| Issue Number | 3 |
| Volume Number | 25 |
| e-ISSN | 14786427 |
| Journal | Natural Product Research |
| Language | English |
| Publisher | Taylor & Francis |
| Publisher Date | 2011-02-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Ascomycota Discipline Natural products Microbiology Plant Extracts Plant Leaves Melia Azedarach Discipline Natural Products Pharmacology Cicer Drug Effects Journal Article Pathogenicity Chemistry Antifungal Agents Plant Diseases |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Plant Science Analytical Chemistry Biochemistry |
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