NDLI: Neuroprotective effect from stem bark extracts of Knema laurina against H2O2- and Aß(1-42)-induced cell death in human SH-SY5Y cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ismail, Norsharina Akhtar, Muhammad Nadeem Ismail, Maznah Zareen, Seema Shah, Syed Adnan Ali Lajis, Nordin Hj Tajuddin, Saiful N.
Description	Country affiliation: Malaysia Author Affiliation: Ismail N ( a Nutricosmeceuticals and Nutrigenomics Programme, Laboratory of Molecular Biomedicine, Institute of Bioscience, University Putra Malaysia , 43400 UPM Serdang , Selangor , Malaysia.)
Abstract	The stem bark extracts of Knema laurina inhibited the hydrogen peroxide (H2O2)- and aggregated amyloid ß-peptide 1-42 length (Aß(1-42))-induced cell death in differentiated SH-SY5Y cells. Exposure of 250 µM H2O2 or 20 µM Aß(1-42) to the cells for 24 h reduced 50% of cell viability. Pretreatment of cells with ethyl acetate extract (EAE) or n-butanol extract (BE) at 300 µg/mL and then exposure to H2O2 protected the cells against the neurotoxic effects of H2O2. Besides, methanolic extract (ME) at 1 and 10 µg/mL exerted neuroprotective effect on Aß(1-42)-induced toxicity to the cells. These results showed that EAE, BE and ME exhibited neuroprotective activities against H2O2- and Aß(1-42)-induced cell death. Flavonoids (3-6) and ß-sitosterol glucoside (8) were isolated from the EAE. Compound 1 was isolated from hexane extract, and compounds 2 and 7 were isolated from dichloromethane extract. All these observations provide the possible evidence for contribution in the neuroprotective effects.
File Format	HTM / HTML
ISSN	14786419
Issue Number	16
Volume Number	29
e-ISSN	14786427
Journal	Natural Product Research
Language	English
Publisher	Taylor & Francis
Publisher Date	2015-01-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Neuroprotective Agents Research Support, Non-u.s. Gov't Discipline Natural products Peptide Fragments Adverse Effects Plant Extracts Humans Hydrogen Peroxide Amyloid Beta-peptides Discipline Natural Products Myristicaceae Plant Bark Drug Effects Journal Article Chemistry Cell Death Cell Line, Tumor
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Plant Science Analytical Chemistry Biochemistry

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